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Methods for Genome Assembly, Haplotype Phasing, and Target Independent Nucleic Acid Detection

  • US 20170314014A1
  • Filed: 07/13/2017
  • Published: 11/02/2017
  • Est. Priority Date: 10/19/2015
  • Status: Active Grant
First Claim
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1. A method of assaying for nucleic acid molecular diversity in a heterogeneous sample, comprisinga) obtaining a stabilized nucleic acid sample comprising a diverse plurality of nucleic acids stabilized such that, for at least one member of the plurality, a first nucleic acid segment and a second nucleic acid segment are held together independent of their common phosphodiester backbone, wherein said phosphodiester backbone is cleaved between said first nucleic acid segment and said second nucleic acid segment;

  • b) tagging said first nucleic acid segment and said second nucleic acid segment such that said first nucleic acid segment and said second nucleic acid segment are identifiable as arising from a common nucleic acid of the diverse plurality of nucleic acids;

    c) sequencing at least an identifiable portion of said first nucleic acid segment and its tag, and an identifiable portion of said second nucleic acid segment and its tag;

    d) assigning said first nucleic acid segment and said second nucleic acid segment to a scaffold corresponding to said tag;

    such that a plurality of segments of said diverse plurality of nucleic acids are assigned to at least one scaffold; and

    e) determining a number corresponding to how many scaffolds are generated;

    wherein the number of scaffolds generated corresponds to the nucleic acid molecular diversity of the heterogeneous sample.

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