SAMPLE ANALYZER AND SAMPLE ANALYZING METHOD THEREOF

• US 20170315046A1
• Filed: 05/24/2016
• Published: 11/02/2017
• Est. Priority Date: 03/18/2016
• Status: Active Grant

• Current Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd. (Excelsior Union Ltd.)
• Original Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd. (Excelsior Union Ltd.)
• Inventors: DU, Xiansuan, DAI, Yong
• Granted Patent: US 10,190,961 B2
• Time in Patent Office: Days
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/014 Reticulocytes
• CPC Class Codes: G01N 2015/018 Platelets
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1402 Data analysis by thresholding or gating operations performed on the acquired signals or stored data
• CPC Class Codes: G01N 21/4738 Diffuse reflection precedence is given to G01N21/55 - G01N21/57 if specular component is taken into consideration, e.g. also for testing fluids, fibrous materials
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 2201/06113 Coherent sources; lasers
• CPC Class Codes: G01N 2201/124 Sensitivity

Abstract

A sample analyzer with an optical detection device and a sample analysis method of the sample analyzer are disclosed. The optical detection device includes a fluid chamber, a light source and a light detector. The fluid chamber includes an illumination zone. An analyte flows through the illumination zone so as to form a sample stream. The light source illuminates the illumination zone to excite cell articles, reacted with a reagent, of the sample stream to emit a light signal. The light detector detects the fluorescent lights and transforms it into an electric signal. The light detector can include a silicon photomultiplier.

Claims

1. A sample analyzer, comprising:

a sample collection device for collecting a sample quantitatively, wherein the sample comprises cell particles;

a reagent supplement device for providing a reagent, wherein the reagent is able to react with the cell particles;

a sample reaction device for receiving the sample from the sample collection device and the reagent from the reagent supplement device, wherein the reagent reacts with the cell particles to generate an analyte;

an analyte delivery device for delivering the analyte for optical measurement; and

an optical measurement device for measuring a light signal generated from the analyte to generate light signal information, wherein the optical measurement device comprises;

a fluid chamber comprising an illumination zone, wherein the analyte from the analyte delivery device flows through the illumination zone to form a sample stream;

a light source for illuminating the illumination zone to generate the light signal from the sample stream; and

a light detector for detecting the light signal and transforming the light signal into the light signal information, wherein the light detector comprises at least one silicon photomultiplier.

2. The sample analyzer of claim 1, wherein the light source comprises a laser generator, the output power of the laser generator is between 1 to 20 mW.

3. The sample analyzer of claim 1, wherein the light source comprises a laser generator, the output power of the laser generator is between 1 to 15 mW.

4. The sample analyzer of claim 1, wherein the silicon photomultiplier comprises a plurality of light sensing units arranged in an array configuration, an illumination area of each light sensing unit is smaller than an imaging area of a single cell particle, a size of the light sensing unit is between 10 μ

m to 50 μ

m.

5. The sample analyzer of claim 1, wherein the silicon photomultiplier comprises a plurality of light sensing units arranged as array configuration, the number of the light sensing units is larger or equal to 500 units;

6. The sample analyzer of claim 1, wherein the silicon photomultiplier comprises a plurality of light sensing units arranged as array configuration, preferably, the number of the light sensing units is larger or equal to 1280 units.

7. The sample analyzer of claim 1, wherein a light sensing area of the silicon photomultiplier is smaller than a threshold value, a distortion of a pulse amplitude of an electrical signal is triggered when the light sensing area of the silicon photomultiplier exceeds the threshold value, the electrical signal is generated according to an amount of dark pulse count overlapping on single one of the cell particles.

8. The sample analyzer of claim 1, wherein a light sensing area of the silicon photomultiplier is between 1-36 mm², the light sensing area is a circle with a diameter between 2 mm to 6 mm.

9. The sample analyzer of claim 1, wherein a light sensing area of the silicon photomultiplier is between 1-36 mm², the light sensing area is a square with a length between 1 mm to 6 mm.

10. The sample analyzer of claim 1, wherein the optical measurement device further comprises:

an optical path, configured between the fluid chamber and the light detector, for converging the light signal to form a facula on a light sensing area of the silicon photomultiplier, the facula is between 50% to 78% of the light sensing area.

11. The sample analyzer of claim 1, further comprising a controller for controlling a reverse bias voltage applied on the silicon photomultiplier to keep an overvoltage between 0 to 5 volt, the overvoltage is a differences between the reverse bias voltage and a breakdown voltage of the silicon photomultiplier.

12. The sample analyzer of claim 11, wherein the controller adjusts the reverse bias voltage applied on the silicon photomultiplier according to different operation modes to control the overvoltage.

13. The sample analyzer of claim 1, further comprising a temperature control device for controlling a temperature of the silicon photomultiplier at a configuration temperature, the configuration temperature is selected between 20°

C. to 40°

C.

14. The sample analyzer of claim 1, further comprising a temperature compensation device for adjusting a reverse bias voltage applied on the silicon photomultiplier according to a temperature of the silicon photomultiplier so as to keep an overvoltage constant;

15. The sample analyzer of claim 14, wherein the temperature compensation device comprises a temperature sensor, a temperature detection circuit, an AD converter, a temperature compensation module, a DA converter, a voltage adjustment circuit and a regulation power supply with an adjustable output, wherein the temperature sensor and the temperature detection circuit detect the temperature of the silicon photomultiplier and generate a temperature signal, the AD converter converters the temperature signal into a digital signal, the temperature compensation module calculates a target value of the reverse bias voltage of the silicon photomultiplier, the controller adjusts a circuit parameter of the voltage adjustment circuit by controlling the DA converter to cause an output voltage of the adjustable regulation power supply to reach the target value of the reverse bias voltage.

16. A sample analysis method for a sample analyzer, comprising:

providing an analyte comprising cell particles treated with a reagent;

providing an optical measurement device comprising a fluid chamber, a light source and a light detector comprising at least one silicon photomultiplier;

when the analyte flows through the fluid chamber and forms a sample stream, illuminating the sample stream flowing through the fluid chamber by the light source to generate a light signal, and transforming the light signal into light signal information after the light detector receives the light signal; and

classifying the cell particles according to the light signal information.

17. The sample analysis method of claim 16, wherein light power of the light source is between 5 to 15 mW.

18. The sample analysis method of claim 16, wherein the cell particles are at least selected from red blood cells, white blood cells and platelets.

19. The sample analysis method of claim 18, wherein the red blood cells comprise nucleated red blood cells and reticulocytes.

20. The sample analysis method of claim 16, wherein the silicon photomultiplier comprises a plurality of light sensing units arranged in an array configuration, an illumination area of each light sensing unit is smaller than an imaging area of a single cell particle.

21. The sample analysis method of claim 16, wherein a reverse bias voltage applied on the silicon photomultiplier is larger than a breakdown voltage.

22. The sample analysis method of claim 21, wherein a first reverse bias voltage is applied on the light sensing units when the cell particles are white blood cells and a second reverse bias voltage is applied on the light sensing unit when the cell particles are reticulocytes, the first reverse bias voltage is smaller than the second reverse bias voltage.