SYSTEM AND METHOD FOR SELECTING AND CULTURING CELLS

• US 20170315121A1
• Filed: 04/27/2017
• Published: 11/02/2017
• Est. Priority Date: 04/29/2016
• Status: Active Grant

• Current Assignee: Fenwal Incorporated (Fresenius SE & Co. KGaA)
• Original Assignee: Fenwal Incorporated (Fresenius SE & Co. KGaA)
• Inventors: Wegener, Christopher J., Olson, Bret M., Schlinker, Alaina, Binninger, Steven, Kadakia, Avnie A., Thompson, Kyle, Wolok, Kyle, Hamilton, Melanie
• Granted Patent: US 10,449,283 B2
• Time in Patent Office: Days
• CPC Class Codes: A61M 1/262 rotating
• CPC Class Codes: A61M 1/265 inducing Taylor vortices
• CPC Class Codes: A61M 1/3618 Magnetic separation
• CPC Class Codes: A61M 1/362 changing physical properties of target cells by binding them to added particles to facilitate their subsequent separation from other cells, e.g. immunoaffinity
• CPC Class Codes: C12M 23/28 disposable or single use
• CPC Class Codes: C12M 29/14 Pressurized fluid
• CPC Class Codes: C12M 33/14 with filters, sieves or membranes
• CPC Class Codes: C12M 41/48 Automatic or computerized control automatic analysis G01N35/00
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

A cell processing system includes at least one processor connectable to a source container filled with a biological fluid, the at least one processor including a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet, one or more containers selectively connected to the first outlet; and, and a magnet. The system also includes a controller coupled to the at least one processor and configured to operate the spinning membrane to receive biological fluid from the source container and to direct the target cells to one of the one or more containers, to pause to permit magnetic particles to be associated with the target cells, and to operate the spinning membrane to receive the contents of one of the one or more containers with the magnet applied to the target cells associated with the magnetic particles.

Claims

1. A cell processing system comprising:

at least one processor connectable to a source container filled with a biological fluid, the at least one processor comprising;

a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet;

one or more containers selectively connected to the first outlet; and

a magnet;

a controller coupled to the at least one processor and configured;

to operate the spinning membrane to receive biological fluid from the source container and to direct the target cells to one of the one or more containers,

to pause to permit magnetic particles to be associated with the target cells,

to operate the spinning membrane to receive the contents of one of he one or more containers with the magnet applied to the target cells associated with the magnetic particles.

2. The cell processing system according to claim 1, wherein the controller is configured to pause to permit a conjugated antibody bead to be added to the one or more containers and to permit the conjugated antibody bead to form conjugated antibody bead-target cell complexes with the target cells.

3. The cell processing system according to claim 2, wherein the controller is configured to operate the spinning membrane to mix the conjugated antibody beads with the target cells,

4. The cell processing system according to claim 1, wherein the controller is configured to pause to permit monoclonal antibodies to be added to the target cells to associate the monoclonal antibodies with the target cells, and to permit magnetic particles to be added to the target cells associated with the monoclonal antibodies to associate the magnetic particles with the target cells.

5. The cell processing system according to claim 4, wherein the controller is configured to operate the spinning membrane to mix the monoclonal antibodies with the target cells, and wherein the controller is configured to operate the spinning membrane to mix the magnetic particles with the target cells associated with the monoclonal antibodies.

6. The cell processing system according to claim 1, further comprising:

a magnetic separator having first and second opposing plates, the magnet associated with the first plate.

at least the first plate translatable relative to the second plate, and

one of the one or more containers disposed between the first and second plates.

7. The cell processing system according to claim 6, wherein the first and second plates are mounted on an axle, the first and second plates being pivotable about the axle to change the inclination of the first and second plates and the second container disposed between the first and second plates.

8. The cell processing system according to claim 1, wherein the at least one processor comprises reusable hardware, and a disposable circuit, the disposable circuit comprising the spinning membrane and the one or more containers.

9. The cell processing system according to claim 8, wherein the disposable circuit is a closed circuit.

10. A cell processing method comprising:

separating a biological fluid into at least two streams, one of he streams including target cells;

associating magnetic particles with the target cells; and

separating the target cells associated with the magnetic particles using a magnetic field.

11. The cell processing method according to claim 10, wherein associating magnetic particles with the target cells comprises adding conjugated antibody beads to the target cells in a container, and incubating the target cells with the conjugated antibody beads.

12. The cell processing method according to claim 11, further comprising subsequently passing the contents of the container through a spinning membrane to mix the contents of the container.

13. The cell processing method according to claim 10, wherein associating magnetic particles with the target cells comprises associating monoclonal antibodies with the target cells, and associating magnetic particles with the monoclonal antibodies associated with the target cells

14. The cell processing method according to claim 13, wherein:

associating monoclonal antibodies with the target cells comprises adding the monoclonal antibodies to the target cells in a container and subsequently passing the contents of the container through a spinning membrane to mix the contents of the container; and

associating magnetic particles with the monoclonal antibodies comprises adding the magnetic particles to the monoclonal antibodies associated with the target cells in the container and subsequently passing the contents of the container through a spinning membrane to mix the contents of the container.

15. The cell processing method according to claim 10, selecting the target cells using a magnetic field comprises disposing a magnet adjacent the target cells associated with magnetic particles.

16. The cell processing method according to claim 15, wherein the target cells associated with magnetic particles are disposed in a container that is disposed between two moveable plates, one of the plates comprising a magnet that is translatable relative to the container.

17. The cell processing method according to claim 16, wherein the two moveable plates are mounted on an axle, the first and second plates being pivotable about the axle.

18. The cell processing method according to claim 10, wherein separating the biological fluid into at least two streams comprises passing the biological fluid through a spinning membrane.

19. The cell processing method according to claim 10, wherein the method is performed within a single closed fluid circuit.