LETTUCE PLANTS COMPRISING RESISTANCE AGAINST NASONOVIA RIBISNIGRI BIOTYPE 1

• US 20170318770A1
• Filed: 10/29/2015
• Published: 11/09/2017
• Est. Priority Date: 10/30/2014
• Status: Active Grant

• Current Assignee: Nunhems BV (BASF SE)
• Original Assignee: Nunhems BV (BASF SE)
• Inventors: SCHAAREMAN, Robert Theodorus Gerardus, THOMAS, Vincent Pierre Andre, VAN DER AREND, Adrianus J. M., RAEDTS, Robert Johannes Martinus, JULIAN RODRIGUEZ, Olga
• Granted Patent: US 10,638,689 B2
• Time in Patent Office: Days
• CPC Class Codes: A01H 1/045 using molecular markers
• CPC Class Codes: A01H 5/12 Leaves
• CPC Class Codes: A01H 6/1472 Lactuca sativa [lettuce]
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8279 for biotic stress resistance, pathogen resistance, disease resistance
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism

Abstract

The present invention relates to the field of lettuce breeding, in particular to Quantitative Trait Loci for resistance against the lettuce aphid Nasonovia ribisnigri biotype Nr:1 and to cultivated lettuce comprising one or more of these Quantitative Trait Loci.

Claims

1. A Lactuca sativa plant comprising an introgression fragment from Lactuca virosa on chromosome 6 and/or on chromosome 7 which comprises a Quantitative Trait Locus that confers resistance against Nasonovia ribisnigri biotype 1 (Nr:

1), and wherein said introgression fragment on chromosome 6 comprises all or part of the region starting at 77 Mb and ending at 161 Mb of chromosome 6 and wherein said introgression fragment on chromosome 7 comprises all or part of the region starting at 203 Mb and ending at 219 Mb of chromosome 7.

2. The plant according to claim 1, comprising both the introgression fragment on chromosome 6 and on chromosome 7.

3. The plant according to claim 1, wherein said introgression fragment on chromosome 6 is detectable by a molecular marker assay which detects at least 1, 2, 3, 4, 5, or more of the following markers:

a) the CC or CT genotype for the Single Nucleotide Polymorphism marker SNP1.23 in SEQ ID NO;

23 or in a sequence comprising substantial sequence identity to SEQ ID NO;

23;

b) the CC or CT genotype for the Single Nucleotide Polymorphism marker SNP_02 in SEQ ID NO;

2 or in a sequence comprising substantial sequence identity to SEQ ID NO;

2;

c) the TT or CT genotype for the Single Nucleotide Polymorphism marker SNP2.24 in SEQ ID NO;

24 or in a sequence comprising substantial sequence identity to SEQ ID NO;

24;

the AA or AC genotype for the Single Nucleotide Polymorphism marker SNP_03 in SEQ ID NO;

3 or in a sequence comprising substantial sequence identity to SEQ ID NO;

3;

d) any wild lettuce genome specific marker, especially L. virosa-genome specific marker, located physically in-between SNP1.23 and SNP_03, or in between SNP_02 and SNP_03, or in between SNP2.24 and SNP_03; and

/or

e) any wild lettuce genome specific marker especially L. virosa-genome specific marker, located within a distance of 10 Mb, preferably within 5 Mb, of any marker selected from SNP1.23, SNP_02, SNP2.24, or SNP_03.

4. The plant according to claim 1, wherein said introgression fragment on chromosome 6 is detectable by a molecular marker assay which detects:

a) the GG or GT genotype for the Single Nucleotide Polymorphism marker VSP1 in SEQ ID NO;

26 or in a sequence comprising substantial sequence identity to SEQ ID NO;

26, or

b) the AA or AC genotype for the Single Nucleotide Polymorphism marker VSP3 in SEQ ID NO;

27 or in a sequence comprising substantial sequence identity to SEQ ID NO;

27.

5. The plant according to claim 1, wherein the introgression fragment on chromosome 7 is detectable by a molecular marker assay which detects at least 1, 2, 3, 4, 5, or more of the following markers:

a) the TT or TC genotype for the Single Nucleotide Polymorphism marker SNP_17 in SEQ ID NO;

17 or in a sequence comprising substantial sequence identity to SEQ ID NO;

17;

b) the TT or TC genotype for the Single Nucleotide Polymorphism marker SNP17.25 in SEQ ID NO;

25 or in a sequence comprising substantial sequence identity to SEQ ID NO;

25;

c) the GG or GC genotype for the Single Nucleotide Polymorphism marker SNP_18 in SEQ ID NO;

18 or in a sequence comprising substantial sequence identity to SEQ ID NO;

18;

d) the GG or GA genotype for the Single Nucleotide Polymorphism marker SNP_19 in SEQ ID NO;

19 or in a sequence comprising substantial sequence identity to SEQ ID NO;

19;

e) any wild lettuce genome specific marker, especially L. virosa-genome specific marker, located physically in-between SNP_17 and SNP_19, or in-between SNP_17 and SNP_18, or in between SNP_17 and SNP17.25, or in between SNP17.25 and SNP_19, or in between SNP17.25 and SNP_18, or in between SNP_18 and SNP_19; and

/or

f) any wild lettuce genome specific marker especially L. virosa-genome specific marker, located within a distance of 12 Mb, 10 Mb, preferably within 5 Mb, of any marker selected from SNP_17, SNP_17.25, SNP_18 and SNP_19.

6. The plant according to claim 1, wherein said introgression fragment on chromosome 7 is detectable by a molecular marker assay which detects:

a) the CC or CA genotype for the Single Nucleotide Polymorphism marker VSP2 in SEQ ID NO;

28 or in a sequence comprising substantial sequence identity to SEQ ID NO;

28, or

b) the GG or GA genotype for the Single Nucleotide Polymorphism marker VSP4 in SEQ ID NO;

29 or in a sequence comprising substantial sequence identity to SEQ ID NO;

29.

7. The plant according to claim 1, wherein said plant comprises resistance against Nasonovia ribisnigri biotype 1 under both free-choice and under non-choice test conditions.

8. The plant according to claim 1, wherein the average number of aphids of biotype 1 on said plant is at most 50% of the average number of aphids of biotype 1 found on a Nr:

1 susceptible control plant, such as lettuce variety Mafalda, when grown under the same conditions.

9. The plant according to claim 1, wherein the average number of aphids of biotype 1 on said plant is less than 10 aphids, while the average number of aphids of biotype 1 on the Nr:

1 susceptible control plant, such as variety Mafalda, is at least 100 aphids, when grown under the same conditions.

10. The plant according to claim 1, wherein the Lactuca sativa plant is loose-leaf lettuce, Romaine lettuce, Crisphead lettuce, Butterhead lettuce, Batavia lettuce or Stem lettuce.

11. The plant according to claim 1, wherein said introgression fragment is a fragment of chromosome 6 and/or 7 as found in L. virosa accession NCIMB42086.

12. Seed from which a plant according to claim 1 can be grown.

13. Leaves or heads of the plant according to claim 1.

14. A progeny plant of the lettuce plant according to claim 1, wherein said progeny plant retains the introgression fragment on chromosome 6 comprising QTL6.1 and/or chromosome 7 comprising QTL7.1.

15. The progeny plant according to claim 14, wherein the progeny plant is produced by one or more of:

selfing, crossing, mutation, double haploid production or transformation.

16. A part of a lettuce plant according to claim 1, wherein the part is selected from the group consisting of:

stems, cuttings, petioles, cotyledons, flowers, anthers, pollen, ovaries, roots, root tips, protoplasts, callus, microspores, stalks, ovules, shoots, seeds, embryos, embryo sacs, cells, buds, leaves, and meristems, and wherein said part comprises said introgression fragment on chromosome 6 and/or 7.