COMPOSITIONS AND METHODS FOR DETECTION OF TRICHOMONAS VAGINALIS
First Claim
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1. A method of detecting Trichomonas vaginalis (TV) in a sample, the method comprising:
- performing an amplifying step comprising contacting the sample with a set of target TV gene primers to produce an amplification product if a target TV gene nucleic acid is present in the sample;
performing a hybridizing step comprising contacting the amplification product with one or more detectable target TV gene probes; and
detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of TV in the sample and wherein the absence of the amplification product is indicative of the absence of TV in the sample;
wherein the set of target TV gene primers comprise a first primer comprising a first oligonucleotide sequence selected from the group consisting of SEQ ID NOs;
1-9, 19-28, 45-48, 55-64, and 80-89 or a complement thereof, and a second primer comprising a second oligonucleotide sequence selected from the group consisting of SEQ ID NOs;
10-13, 29-38, 49-52, 65-74, and 90-99, or a complement thereof; and
wherein the one or more detectable target TV gene probes comprises a third oligonucleotide sequence selected from the group consisting of SEQ ID NOs;
14-18, 39-44, 53-54, 75-79, and 100-105, or the complement thereof.
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Abstract
Methods for the rapid detection of the presence or absence of Trichomonas vaginalis (TV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the target TV gene, along with kits are provided that are designed for the detection of TV.
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Citations
11 Claims
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1. A method of detecting Trichomonas vaginalis (TV) in a sample, the method comprising:
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performing an amplifying step comprising contacting the sample with a set of target TV gene primers to produce an amplification product if a target TV gene nucleic acid is present in the sample; performing a hybridizing step comprising contacting the amplification product with one or more detectable target TV gene probes; and detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of TV in the sample and wherein the absence of the amplification product is indicative of the absence of TV in the sample; wherein the set of target TV gene primers comprise a first primer comprising a first oligonucleotide sequence selected from the group consisting of SEQ ID NOs;
1-9, 19-28, 45-48, 55-64, and 80-89 or a complement thereof, and a second primer comprising a second oligonucleotide sequence selected from the group consisting of SEQ ID NOs;
10-13, 29-38, 49-52, 65-74, and 90-99, or a complement thereof; andwherein the one or more detectable target TV gene probes comprises a third oligonucleotide sequence selected from the group consisting of SEQ ID NOs;
14-18, 39-44, 53-54, 75-79, and 100-105, or the complement thereof. - View Dependent Claims (2, 3, 4, 5)
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6. A kit for detecting a nucleic acid of Trichomonas vaginalis (TV) comprising:
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a first primer comprising a first oligonucleotide sequence selected from the group consisting of SEQ ID NOs;
1-9, 19-28, 45-48, 55-64, and 80-89, or a complement thereof;a second primer comprising a second oligonucleotide sequence selected from the group consisting of SEQ ID NOs;
10-13, 29-38, 49-52, 65-74, and 90-99, or a complement thereof; anda fluorescently detectably labeled probe comprising a third oligonucleotide sequence selected from the group consisting of SEQ ID NOs;
14-18, 39-44, 53-54, 75-79, and 100-105, or a complement thereof, the detectably labeled probe configured to hybridize to an amplicon generated by the first primer and the second primer. - View Dependent Claims (7, 8, 9, 10, 11)
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Specification