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QUANTITATIVE MASSIVELY PARALLEL PROTEOMICS

  • US 20180024139A1
  • Filed: 07/21/2017
  • Published: 01/25/2018
  • Est. Priority Date: 07/22/2016
  • Status: Active Grant
First Claim
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1. A method of identifying an expression profile in a single cell of a population of cells, comprising:

  • a) contacting a population of cells with a plurality of antibodies under conditions that promote specific binding of the antibodies to target antigens of the cells, wherein each antibody in the plurality is capable of binding to a unique target antigen in one or more cells, wherein each antibody is conjugated to an oligonucleotide, and wherein the oligonucleotide comprises a unique molecular identifier sequence and an antibody identifier sequence corresponding to the unique target antigen;

    b) separating single cells of the population into individual compartments;

    c) amplifying the unique molecular identifier sequences and antibody identifier sequences in each individual compartment; and

    d) analyzing the amplified unique molecular identifier sequences and antibody identifier sequences to identify the expression profile of each single cell.

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