Low Sequence Bias Single-Stranded DNA Ligation
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Abstract
The invention provides compositions and methods for ligating single stranded nucleic acids wherein the ligation is based on fast, efficient, and low-sequence bias hybridization of an acceptor molecule with a donor molecule. In one embodiment, the structure of the donor molecule comprises a stem-loop intramolecular nucleotide base pairing (i.e., hairpin) and a 3′-overhang region such that the overhang is able to hybridize to nucleotides present in the 3′ end of the acceptor molecule.
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20 Claims
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1-11. -11. (canceled)
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12. A composition comprising a donor nucleic acid molecule, wherein the molecule comprises a stem-loop structure and a 3′
- overhang, further wherein the molecule comprises a continuous primer binding site that encompasses a portion of the stem and a portion of the loop structure.
- View Dependent Claims (13, 14, 15, 16, 17)
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18. A kit comprising a donor nucleic acid molecule, wherein the molecule comprises a stem-loop structure and a 3′
- overhang, further wherein the molecule comprises a continuous primer binding site that encompasses a portion of the stem and a portion of the loop structure.
- View Dependent Claims (19, 20)
Specification