AMPLIFICATION AND ANALYSIS OF WHOLE GENOME AND WHOLE TRANSCRIPTOME LIBRARIES GENERATED BY A DNA POLYMERIZATION PROCESS
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
33 Citations
73 Claims
-
1-51. -51. (canceled)
-
52. A kit comprising:
-
a container; and a population of polynucleotide primers present in the container, wherein the primers of the population; (a) have sequences configured to be non-self-complementary and non-complementary to each other; (b) comprise a variable region and a constant region, wherein the constant region is positioned 5′
to the variable region; and(c) have sequences that are 70% or more comprised of two non-complementary and non-self-complementary nucleotides. - View Dependent Claims (53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73)
-
Specification