SELECTIVE OXIDATION OF 5-METHYLCYTOSINE BY TET-FAMILY PROTEINS
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Abstract
The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.
19 Citations
122 Claims
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1-96. -96. (canceled)
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97. A method comprising:
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(a) obtaining an isolated cell; and (b) regulating activity of a TET enzyme in said isolated cell, wherein said regulating comprises contacting said isolated cell with a TET inhibitor, wherein said contacting causes a decreased conversion of 5-methylated cytosine to 5-hydroxymethylated cytosine in said isolated cell, wherein said TET enzyme comprises a TET1 enzyme, a TET2 enzyme, a TET3 enzyme, a CXXC4 enzyme, or any combination thereof. - View Dependent Claims (98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 119, 120, 121, 122)
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115. The method of claim 115, wherein said nucleic acid comprises RNA or DNA.
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116. The method of claim 116, wherein said nucleic acid comprises RNA.
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117. The method of claim 117, wherein said RNA is siRNA.
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118. The method of claim 118, wherein said siRNA is a TET1 siRNA, a TET2 siRNA, or a TET3 siRNA.
Specification