SELECTIVE OXIDATION OF 5-METHYLCYTOSINE BY TET-FAMILY PROTEINS
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Abstract
The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.
20 Citations
118 Claims
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1-96. -96. (canceled)
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97. A method comprising:
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(a) obtaining an isolated cell; and (b) regulating a TET enzyme activity in said isolated cell, wherein said regulating comprises contacting said isolated cell with a TET activator, wherein said contacting causes an increased conversion of 5-methylated cytosine to 5-hydroxymethylated cytosine in said isolated cell, wherein said TET enzyme comprises a TET1 enzyme, a TET2 enzyme, a TET3 enzyme, a CXXC4 enzyme, or any combination thereof. - View Dependent Claims (98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118)
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Specification