ELECTROPHORESIS RUNNING TANK ASSEMBLY

• US 20180045677A1
• Filed: 10/24/2017
• Published: 02/15/2018
• Est. Priority Date: 04/01/2014
• Status: Active Grant

• Current Assignee: CC IMEX
• Original Assignee: CC IMEX
• Inventors: Walker, Winston Glenn, Chan, Richard, Wong, Rita M., Chan, Ramey K., Cheng, Po Ling
• Granted Patent: US 10,641,731 B2
• Time in Patent Office: Days
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

An electrophoresis running tank assembly uses two opposed rows of LEDs to illuminate DNA-containing gel on a transparent tray positioned between the rows. A respective cylindrical lens is positioned horizontally between each row and a respective edge of the tray. The optical axis of the illumination light is midway between a bottom surface of the gel tray and a top surface of the gel.

Claims

1. Assembly for electrophoresis, comprising:

at least one tank formed with a gel tray platform including a top surface configured for holding at least one gel tray containing gel;

at least an anode reservoir on a first side of the gel tray platform and at least a cathode reservoir on a second side of the gel tray platform, both reservoirs configured for holding buffer during electrophoresis;

at least one anode immersed in the anode reservoir and at least one cathode immersed in the cathode reservoir;

a first lens engaged with an opening of a housing supporting the tank, the first lens being elongated in a horizontal dimension, the first lens substantially filling the opening, plural first light emitting diodes (LED) arranged in a horizontal row along a first printed circuit board (PCB) comprising at least one connection to a power source to energize the first LEDs, the first PCB being mounted outboard of the first lens, the first LEDs being recessed radially outward of the inner surface and the first lens being located between the first PCB and a first side of the tank.

2. The assembly of claim 1, wherein the first lens is formed on a parallelepiped-shaped transparent block.

3. The assembly of claim 2, comprising a lens seal be disposed between an outside surface of a side wall and the block.

4. The assembly of claim 1, wherein the PCB comprises a skirt to spread and dissipate heat generated when the first LEDs are energized.

5. The assembly of claim 1, comprising a second lens engaged with an opening of a housing supporting the tank, the second lens being elongated in a horizontal dimension, the second lens substantially filling the opening, plural second light emitting diodes (LED) arranged in a horizontal row along a second printed circuit board (PCB) comprising at least one connection to a power source to energize the second LEDs, the second PCB being mounted outboard of the second lens, the second LEDs being recessed radially outward of the inner surface and the second lens being located between the second PCB and a second side of the tank, the first side of the tank being opposite the second side of the tank.

6. The assembly of claim 1, comprising the gel tray, the gel tray comprising a clear bottom wall defining a bottom surface, the bottom wall supporting a gel defining a top gel surface, the gel tray being positioned on the top surface of the gel tray platform, the first lens focusing light from the first LEDs toward a line midway between the top surface of the gel and the bottom surface of the gel tray.

7. The assembly of claim 6, wherein the top surface of the gel tray platform is at least partially speculative and opaque.

8. The assembly of claim 7, wherein the gel tray is made of clear plastic such that a bottom of the gel tray in cooperation with the at least partially speculative and reflective top surface of the gel tray platform establishes a light pipe to excite stain associated with DNA in the gel.

9. Assembly for electrophoresis, comprising:

at least one tank formed with a gel tray platform including a top surface configured for holding at least one gel or gel tray containing gel;

at least an anode reservoir and at least a cathode reservoir for holding buffer during electrophoresis of a substance in the gel;

at least one anode immersed in the anode reservoir and at least one cathode immersed in the cathode reservoir,

a first lens engaged with an opening of a housing supporting the tank, the first lens being elongated in a horizontal dimension, the first lens substantially filling the opening, plural first light emitting diodes (LED) arranged in a horizontal row along a first printed circuit board (PCB) comprising at least one connection to a power source to energize the first LEDs, the first PCB being mounted outboard of the first lens, the first LEDs being recessed radially outward of the inner surface and the first lens being located between the first PCB and a first side of the tank.

10. The assembly of claim 9, wherein the first lens is formed on a parallelepiped-shaped transparent block.

11. The assembly of claim 9, comprising a second lens engaged with an opening of a housing supporting the tank, the second lens being elongated in a horizontal dimension, the second lens substantially filling the opening, plural second light emitting diodes (LED) arranged in a horizontal row along a second printed circuit board (PCB) comprising at least one connection to a power source to energize the second LEDs, the second PCB being mounted outboard of the second lens, the second LEDs being recessed radially outward of the inner surface and the second lens being located between the second PCB and a second side of the tank, the first side of the tank being opposite the second side of the tank.

12. The assembly of claim 9, comprising the gel tray, the gel tray comprising a clear bottom wall defining a bottom surface, the bottom wall supporting a gel defining a top gel surface, the gel tray being positioned on the top surface of the gel tray platform, the first lens focusing light from the first LEDs toward a line midway between the top surface of the gel and the bottom surface of the gel tray, wherein the top surface of the gel tray platform is at least partially speculative and opaque such that a bottom of the gel tray in cooperation with the at least partially speculative and reflective top surface of the gel tray platform establishes a light pipe to excite stain associated with DNA in the gel.

13. Assembly for electrophoresis, comprising:

at least one gel tray including gel;

at least one support including a top surface configured for holding the at least one gel tray;

at least an anode reservoir and at least a cathode reservoir for holding buffer during electrophoresis of the gel;

at least one anode immersed in the anode reservoir and at least one cathode immersed in the cathode reservoir;

at least one light source;

the gel tray comprising a clear bottom wall defining a bottom surface, the bottom wall supporting the gel, the gel defining a top gel surface, the gel tray being positioned on the top surface of the support, light from the at least one light source propagating along a plane midway between the top surface of the gel and the bottom surface of the gel tray, wherein the top surface of the support is at least partially speculative and opaque such that a bottom of the gel tray in cooperation with the at least partially speculative and reflective top surface of the support promotes excitation of stain associated with a substance in the gel.

14. The assembly of claim 13, wherein the at least one light source comprises at least plural first light emitting diodes (LED), the assembly comprising at least a first lens engaged with an opening of a housing supporting the tank, the first lens being elongated in a horizontal dimension, the first lens substantially filling the opening, the plural first LEDs being arranged in a horizontal row along a first printed circuit board (PCB) comprising at least one connection to a power source to energize the first LEDs, the first PCB being mounted outboard of the first lens, the first LEDs being recessed radially outward of the inner surface and the first lens being located between the first PCB and a first side of the tank.

15. The assembly of claim 13, wherein the first lens is formed on a parallelepiped-shaped transparent block, wherein faces of the first LEDs are positioned against the block.

16. The assembly of claim 13, comprising a second lens engaged with an opening of a housing supporting the tank, the second lens being elongated in a horizontal dimension, the second lens substantially filling the opening, plural second light emitting diodes (LED) arranged in a horizontal row along a second printed circuit board (PCB) comprising at least one connection to a power source to energize the second LEDs, the second PCB being mounted outboard of the second lens, the second LEDs being recessed radially outward of the inner surface and the second lens being located between the second PCB and a second side of the tank, the first side of the tank being opposite the second side of the tank.

17. The assembly of claim 13, comprising a filter configured to filter light from the at least one light source from an observer of the gel while allowing emitted wavelengths from the stain to pass through to the observer.

18. The assembly of claim 17, wherein the filter is established by a hood with a top of filter material for mounting a camera.

19. The assembly of claim 18, wherein at least one side of the hood is formed with filter material to allow observation simultaneously while running the gel.