NUCLEIC ACID CLASSIFICATION
First Claim
1. A method for classifying a target nucleic acid, the method comprising:
- exposing, in a test system, one or more capture probes to the target nucleic acid, the one or more capture probes being attached to a surface;
establishing a first hybridization condition in the test system;
determining a first degree of hybridization of the one or more capture probes with the target nucleic acid under the first hybridization condition;
establishing a second hybridization condition in the test system;
determining a second degree of hybridization of the one or more capture probes with the target nucleic acid under the second hybridization condition; and
classifying the target nucleic acid by comparing the first and the second degrees of hybridization.
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Accused Products
Abstract
A method and system for classifying a target nucleic acid includes exposing, in a test system, one or more capture probes to the target nucleic acid. The one or more capture probes is attached to a surface. A first hybridization condition is established in the test system. A first degree of hybridization of the one or more capture probes with the target nucleic acid under the first hybridization condition is determined. A second hybridization condition in the test system is established. A second degree of hybridization of the one or more capture probes with the target nucleic acid under the second hybridization condition is determined and the target nucleic acid is classified by comparing the first and the second degrees of hybridization.
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Citations
39 Claims
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1. A method for classifying a target nucleic acid, the method comprising:
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exposing, in a test system, one or more capture probes to the target nucleic acid, the one or more capture probes being attached to a surface; establishing a first hybridization condition in the test system; determining a first degree of hybridization of the one or more capture probes with the target nucleic acid under the first hybridization condition; establishing a second hybridization condition in the test system; determining a second degree of hybridization of the one or more capture probes with the target nucleic acid under the second hybridization condition; and classifying the target nucleic acid by comparing the first and the second degrees of hybridization. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for determining an optimal density of capture probes, the method comprising:
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classifying a target nucleic acid; and determining the optimal density of capture probes on a test system, wherein determining comprises; exposing, in a test system, at a hybridization condition, a first set of capture probes to the target nucleic acid, the first set of capture probes being attached to a first surface; determining a first association rate at the first surface; exposing, in the test system, at the hybridization condition, a second set of capture probes to the target nucleic acid, the second set of capture probes being attached to a second surface and having a density different from first set of capture probes at the first surface; determining a second association rate at the second surface; and comparing the first association rate with the second association rate. - View Dependent Claims (11, 12, 13, 14)
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15. A test system for classifying a target nucleic acid, the test system comprising:
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one or more capture probes; a detector configured to determine a degree of hybridization of the one or more capture probes with the target nucleic acid; a non-transitory computer readable medium storing instructions that, when executed by a control system, causes the control system to perform operations comprising; establishing a first hybridization condition in the test system; determining, using the detector, a first degree of hybridization of the one or more capture probes with the target nucleic acid under the first hybridization condition; establishing a second hybridization condition in the test system; determining, using the detector, a second degree of hybridization of the one or more capture probes with the target nucleic acid under the second hybridization condition; and classifying the target nucleic acid by comparing the first and the second degrees of hybridization. - View Dependent Claims (16, 17, 18, 19, 20, 22, 23, 24, 25, 26, 27, 28, 29)
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21. A method for classifying a target nucleic acid, the method comprising:
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exposing, in a test system, one or more capture probes to the target nucleic acid, the one or more capture probes being attached to a detector; measuring a first mass of the target nucleic acid hybridized with the one or more capture probes; modifying the target nucleic acid to generate a second mass; measuring the second mass of the target nucleic acid hybridized with the one or more capture probes; and classifying the target nucleic acid based on comparing the first mass with the second mass.
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30. A method for performing sequence analysis on a target nucleic acid, the method comprising:
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exposing, in a test system, the target nucleic acid to a polymerase that includes a target nucleotide targeted to a target nucleobase on the target nucleic acid, the target nucleic acid being attached to a surface; and determining a mass shift of the target nucleic acid resulting from the exposure of the target nucleic acid to the polymerase. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39)
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Specification