METHODS FOR PERFORMING SPATIAL PROFILING OF BIOLOGICAL MATERIALS
First Claim
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1. A method comprising:
- a) contacting a biological sample comprising a plurality of biological molecules with a spatial barcode array, wherein the spatial barcode array comprises a plurality of oligonucleotides attached thereto, wherein each of the plurality of oligonucleotides comprises a barcode sequence that identifies a location of the plurality of oligonucleotides on the spatial barcode array;
b) attaching the plurality of oligonucleotides to the plurality of biological molecules to generate a plurality of tagged biological molecules;
c) sequencing at least a portion of the plurality of tagged biological molecules; and
d) determining a location of the plurality of biological molecules within the biological sample based on the barcode sequence attached to the tagged biological molecules.
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Abstract
Provided herein are methods and compositions for profiling the spatial distribution of a wide variety of biological molecules in a sample. The methods and compositions are suited for spatial labeling and sequencing of biological molecules (e.g., nucleic acids, proteins) in a biological sample.
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52 Claims
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1. A method comprising:
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a) contacting a biological sample comprising a plurality of biological molecules with a spatial barcode array, wherein the spatial barcode array comprises a plurality of oligonucleotides attached thereto, wherein each of the plurality of oligonucleotides comprises a barcode sequence that identifies a location of the plurality of oligonucleotides on the spatial barcode array; b) attaching the plurality of oligonucleotides to the plurality of biological molecules to generate a plurality of tagged biological molecules; c) sequencing at least a portion of the plurality of tagged biological molecules; and d) determining a location of the plurality of biological molecules within the biological sample based on the barcode sequence attached to the tagged biological molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method comprising:
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a) contacting a biological sample comprising a plurality of biological molecules with a spatial barcode array, wherein the spatial barcode array comprises a plurality of oligonucleotides attached thereto, wherein each of the plurality of oligonucleotides comprises a barcode sequence that identifies a location of the plurality of oligonucleotides on the spatial barcode array; b) attaching the plurality of oligonucleotides to a signal sequence associated with each of the plurality of biological molecules to generate a plurality of tagged signal sequences; c) sequencing at least a portion of the plurality of tagged signal sequences; and d) determining a location of the plurality of biological molecules within the biological sample based on the barcode sequence attached to the plurality of tagged signal sequences. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
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Specification