Electrochemical Test Sensor

• US 20180059042A1
• Filed: 11/01/2017
• Published: 03/01/2018
• Est. Priority Date: 12/10/2007
• Status: Active Grant

• Current Assignee: Ascensia Diabetes Care Holdings AG (PHC Holdings Corporation)
• Original Assignee: Ascensia Diabetes Care Holdings AG (PHC Holdings Corporation)
• Inventors: Zhu, Boru, Henley, Jr., Herbert
• Granted Patent: US 11,442,035 B2
• Time in Patent Office: Days
• CPC Class Codes: B05C 5/0225 characterised by flow controlling means, e.g. valves, located proximate the outlet B05C5/0258, B05C5/0275 take precedence; supply valves upstream the coating head B05C11/1036
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/128 Microapparatus
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A method of depositing reagent on an electrochemical test sensor adapted to determine information relating to an analyte includes providing a base and forming an electrode pattern on the base. The method further includes depositing the reagent on at least the electrode pattern using a reagent-dispensing system. The reagent-dispensing system applies mechanical force to the reagent in the reagent-dispensing system to assist in providing a wet reagent droplet on at least the electrode pattern.

Claims

1-30. -30. (canceled)

31. An electrochemical test sensor comprising a base, a lid, a plurality of electrodes and a dried reagent, the dried reagent comprise an enzyme, a mediator, a cellulose polymer and a multivalent salt such that dried reagent has a uniformity in which the ratio of the thinnest point to the thickest point is greater than about 0.2, the enzyme including glucose dehydrogenase, the mediator including a 3-phenylimino-3H-phenothiazine or a 3-phenylimino-3H-phenoxazine.

32. The electrochemical test sensor of claim 31, wherein the enzyme includes flavin adenine dinucleotide-glucose dehydrogenase (FAD-GDH).

33. The electrochemical test sensor of claim 31, wherein the multivalent salt includes a sulfate or a phosphate.

34. The electrochemical test sensor of claim 33, wherein the multivalent salt includes sodium phosphate.

35. The electrochemical test sensor of claim 31, wherein the multivalent salt has a concentration greater than 40 mM in the reagent solution.

36. The electrochemical test sensor of claim 35, wherein the multivalent salt has a concentration greater than 50 mM in the reagent solution.

37. The electrochemical test sensor of claim 36, wherein the multivalent salt has a concentration greater than 75 mM in the reagent solution.

38. The electrochemical test sensor of claim 31, wherein the cellulose polymer is hydroxyethyl cellulose.

39. The electrochemical test sensor of claim 31, wherein the dried reagent has a uniformity in which the ratio of the thinnest point to the thickest point is greater than 0.3.

40. The electrochemical test sensor of claim 39, wherein the dried reagent has a uniformity in which the ratio of the thinnest point to the thickest point is greater than about 0.4.

41. The electrochemical test sensor of claim 31 further including a polyelectrolyte including at least 0.1 wt. % of the reagent solution.

42. The electrochemical test sensor of claim 41, wherein the polyelectrolyte includes carboxymethyl cellulose (CMC), homopolymer and copolymers of arylic acid, malaic acid, styrenesulfonic acid, vinylsulfonic acid, copolymers or salts thereof.

43. The electrochemical test sensor of claim 41, wherein the reagent solution includes at least about 0.3 wt. % polyelectrolytes.

44. The electrochemical test sensor of claim 43, wherein the reagent solution includes at least about 0.4 wt. % polyelectrolytes.

45. An electrochemical test sensor comprising a base, a lid, a plurality of electrodes and a dried reagent, the dried reagent comprise an enzyme, a mediator, a cellulose polymer and a sufficient amount of a multivalent salt such that dried reagent has a uniformity in which the ratio of the thinnest point to the thickest point is greater than about 0.2, the multivalent salt including sodium phosphate, the enzyme including flavin adenine dinucleotide-glucose hydrogenase (FAD-GDH), the mediator including a 3-phenylimino-3H-phenothiazine or a 3-phenylimino-3H-phenoxazine.

46. The electrochemical test sensor of claim 45, wherein the multivalent salt has a concentration greater than 40 mM in the reagent solution.

47. The electrochemical test sensor of claim 46, wherein the multivalent salt has a concentration greater than 50 mM in the reagent solution.

48. The electrochemical test sensor of claim 45, wherein the cellulose polymer is hydroxyethyl cellulose.

49. The electrochemical test sensor of claim 45, wherein the dried reagent has a uniformity in which the ratio of the thinnest point to the thickest point is greater than about 0.4.

50. The electrochemical test sensor of claim 45, wherein the reagent solution includes at least about 0.3 wt. % polyelectrolytes.