Hydrophobic Nucleic Acid Salts As Security Markers

• US 20180094200A1
• Filed: 04/20/2016
• Published: 04/05/2018
• Est. Priority Date: 04/20/2015
• Status: Active Application

• Current Assignee: APDN (B.V.I.) Inc. (Applied DNA Sciences Incorporated)
• Original Assignee: APDN (B.V.I.) Inc. (Applied DNA Sciences Incorporated)
• Inventors: Szczepanik, Maciej B.
• Application Number:
  US15/562,495
• Publication Number:
  US 20180094200A1
• Time in Patent Office: Days
• CPC Class Codes: C10L 1/003 Marking, e.g. coloration by addition of pigments
• CPC Class Codes: C10L 1/10 containing additives
• CPC Class Codes: C10L 2230/16 Tracers which serve to track or identify the fuel component or fuel composition
• CPC Class Codes: C10L 2270/026 for diesel engines, e.g. automobiles, stationary, marine
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6804 Nucleic acid analysis using immunogens immunoassay G01N33/53
• CPC Class Codes: G01N 33/02 Food
• CPC Class Codes: G01N 33/28 Oils , i.e. hydrocarbon liquids edible oils or edible fats G01N33/03
• CPC Class Codes: G01N 33/44 Resins; Plastics; Rubber; Leather

Abstract

The invention provides a method of marking a hydrophobic medium with a nucleic acid marker, the method includes: providing a trialkylammonium salt of the nucleic acid marker, a tetraalkylphosphonium salt of the nucleic acid marker or a tetraarylphosphonium salt of the nucleic acid marker; and incorporating the trialkylammonium salt of the nucleic acid marker, the tetraalkylphosphonium salt of the nucleic acid marker or the tetraarylphosphonium salt of the nucleic acid marker into the hydrophobic medium. The hydrophobic medium can be authenticated after shipping or recovery from the stream of commerce by detecting the nucleic acid marker in a sample of the hydrophobic medium.

Claims

1. A method of marking a hydrophobic medium with a nucleic acid marker, the method comprising:

providing a trialkylammonium salt of the nucleic acid marker, a tetraalkylphosphonium salt of the nucleic acid marker or a tetraarylphosphonium salt of the nucleic acid marker; and

incorporating the trialkylammonium salt of the nucleic acid marker, the tetraalkylphosphonium salt of the nucleic acid marker or the tetraarylphosphonium salt of the nucleic acid marker into the hydrophobic medium.

2. The method of claim 1, wherein the sequence of the nucleic acid marker encodes information specific to the hydrophobic medium.

3. The method of claim 1, wherein the nucleic acid marker comprises DNA.

4. The method of claim 3, wherein the DNA comprises a single stranded molecule of from about 10 to about 10,000 bases.

5. The method of claim 4, wherein the DNA is a single stranded molecule of from about 15 to about 1,000 bases.

6. The method of claim 3, wherein the DNA is a double stranded molecule of from about 10 to about 10,000 base pairs.

7. The method of claim 6, wherein the DNA is a double stranded molecule of from about 15 to about 1,000 base pairs.

8. The method of claim 1, wherein the nucleic acid marker is a non-natural sequence.

9. A method of authenticating a hydrophobic medium with a nucleic acid marker, the method comprising:

providing a trialkylammonium salt of the nucleic acid marker, a tetraalkylphosphonium salt of the nucleic acid marker or a tetraarylphosphonium salt of the nucleic acid marker; and

incorporating the trialkylammonium salt of the nucleic acid marker, the tetraalkylphosphonium salt of the nucleic acid marker or the tetraarylphosphonium salt of the nucleic acid marker into the hydrophobic medium; and

detecting the nucleic acid marker and thereby authenticating the hydrophobic medium.

10. The method of claim 9, wherein the sequence of the nucleic acid marker encodes information specific to the hydrophobic medium.

11. The method of claim 9, wherein the nucleic acid marker comprises DNA.

12. The method of claim 11, wherein the DNA is a single stranded molecule of from about 10 to about 10,000 bases.

13. The method of claim 12, wherein the DNA is a single stranded molecule of from about 15 to about 1000 bases.

14. The method of claim 11, wherein the DNA is a double stranded molecule of from about 10 to about 10,000 base pairs.

15. The method of claim 14, wherein the DNA is a double stranded molecule of from about 15 to about 1000 base pairs.

16. The method of claim 9, wherein the nucleic acid marker is a non-natural sequence.

17. The method of claim 9, wherein the hydrophobic medium is a petroleum-derived material.

18. The method of claim 9, wherein the hydrophobic medium is a petroleum-derived material is an oil, a crude oil, a fuel, a diesel fuel, a polymer, a building material, an asphalt, a medicine, a cosmetic, or a plastic.

19. The method of claim 9, wherein the hydrophobic medium is an oil comprising food material, medicinal material or cosmetic material.

20. The method of claim 9, wherein the hydrophobic medium is a gel comprising food material, medicinal material or cosmetic material.

21. A method of authenticating and tracking a hydrophobic medium with a nucleic acid marker, the method comprising:

providing a trialkylammonium salt of the nucleic acid marker, a tetraalkylphosphonium salt of the nucleic acid marker or a tetraarylphosphonium salt of the nucleic acid marker;

incorporating the trialkylammonium salt of the nucleic acid marker, the tetraalkylphosphonium salt of the nucleic acid marker or the tetraarylphosphonium salt of the nucleic acid marker into the hydrophobic medium;

introducing the trialkylammonium salt of the nucleic acid marker, the tetraalkylphosphonium salt of the nucleic acid marker or the tetraarylphosphonium salt of the nucleic acid marker into the stream of commerce;

providing a sample of the hydrophobic medium from the stream of commerce; and

detecting the nucleic acid marker in the sample of the hydrophobic medium and thereby authenticating the hydrophobic medium.