METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
First Claim
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1. A library of gel beads for barcoding nucleic acid molecules, comprising:
- a plurality of gel beads that are degradable upon application of a stimulus, wherein said plurality of gel beads comprises a plurality of barcode sequences that are different across said plurality of gel beads,wherein a given gel bead of said plurality of gel beads comprises a plurality of nucleic acid molecules,wherein each of said plurality of nucleic acid molecules comprises a barcode sequence from said plurality of barcode sequences and a unique molecular sequence, wherein (i) said barcode sequence is constant across said plurality of nucleic acid molecules, and (ii) said unique molecular sequence varies across said plurality of nucleic acid molecules.
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
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30 Claims
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1. A library of gel beads for barcoding nucleic acid molecules, comprising:
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a plurality of gel beads that are degradable upon application of a stimulus, wherein said plurality of gel beads comprises a plurality of barcode sequences that are different across said plurality of gel beads, wherein a given gel bead of said plurality of gel beads comprises a plurality of nucleic acid molecules, wherein each of said plurality of nucleic acid molecules comprises a barcode sequence from said plurality of barcode sequences and a unique molecular sequence, wherein (i) said barcode sequence is constant across said plurality of nucleic acid molecules, and (ii) said unique molecular sequence varies across said plurality of nucleic acid molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A composition for barcoding a nucleic acid molecule, comprising:
a gel bead that is degradable upon application of a stimulus, wherein said gel bead comprises a plurality of nucleic acid molecules, wherein each of said plurality of nucleic acid molecules comprises a barcode sequence and a unique molecular sequence, wherein (i) said barcode sequence is constant across said plurality of nucleic acid molecules, and (ii) said unique molecular sequence varies across said plurality of nucleic acid molecules. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
Specification