MEASURING CONCENTRATION OF ANALYTES IN LIQUID SAMPLES USING SURFACE-ENHANCED RAMAN SPECTROSCOPY
First Claim
1. A method of measuring the amount of an analyte in water, the method comprising the steps of:
- obtaining a sample of water of known volume containing an analyte;
mixing a known amount of an isotopologue with the sample;
mixing Raman-scattering nanoparticles with the sample;
producing a Raman spectrum of the mixture; and
,performing a ratiometric analysis of peaks or bands within the Raman spectrum corresponding to the analyte and the isotopologue within the Raman spectrum to quantify the analyte.
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Accused Products
Abstract
A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port and a cartridge for input to the cartridge receiving port. An optical detection system in the housing is capable of providing an illuminated electric field useful for Raman spectroscopy. The cartridge may have a sample well. The sample well is loaded with a mixture of water containing the analyte, Raman-scattering nanoparticles and a calibration solution. The calibration solution contains an analogue of the analyte differing in its Raman response, for example an isotope of the analyte. Optionally, a chemical compound capable of increasing interaction between the analyte and the nanoparticles may be added.
6 Citations
23 Claims
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1. A method of measuring the amount of an analyte in water, the method comprising the steps of:
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obtaining a sample of water of known volume containing an analyte; mixing a known amount of an isotopologue with the sample; mixing Raman-scattering nanoparticles with the sample; producing a Raman spectrum of the mixture; and
,performing a ratiometric analysis of peaks or bands within the Raman spectrum corresponding to the analyte and the isotopologue within the Raman spectrum to quantify the analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 19, 20, 21, 22, 23)
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17. A method of measuring the amount of an analyte in water, the method comprising the steps of:
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obtaining a sample of water of known volume containing an analyte; reacting the analyte with a compound to consume the compound or produce a second compound; mixing a known amount of an isotopologue with the reaction product; mixing Raman-scattering nanoparticles with the reaction product; producing a Raman spectrum of the mixture; and
,performing a ratiometric analysis of peaks or bands within the Raman spectrum corresponding to the compound or second compound or both and the isotopologue within the Raman spectrum to quantify the analyte. - View Dependent Claims (18)
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Specification