Method Of Nucleic Acid Fragment Detection
First Claim
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1. A method of nucleic acid fragment detection, the method comprising:
- capturing a target nucleic acid fragment by an oligonucleotide probe to form a section of hybridised double strand, the oligonucleotide probe having an identification sequence and a reproducible sequence, the identification sequence being complementary to the target nucleic acid fragment;
removing the hybridised double strand section to expose the reproducible sequence of the oligonucleotide probe;
producing repeats of the reproducible sequence; and
labelling the repeats of the reproducible sequence by a detection probe.
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Abstract
A method of nucleic acid fragment detection includes capturing a target nucleic acid fragment by an oligonucleotide probe to form a hybridised double strand. The oligonucleotide probe has an identification sequence complementary to the target nucleic acid fragment and a reproducible sequence. The hybridised double strand is removed to expose the reproducible sequence of the oligonucleotide probe. The repeats of the reproducible sequence are produced. The repeats of the reproducible sequence are labelled by a detection probe for identification and quantitation.
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14 Claims
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1. A method of nucleic acid fragment detection, the method comprising:
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capturing a target nucleic acid fragment by an oligonucleotide probe to form a section of hybridised double strand, the oligonucleotide probe having an identification sequence and a reproducible sequence, the identification sequence being complementary to the target nucleic acid fragment; removing the hybridised double strand section to expose the reproducible sequence of the oligonucleotide probe; producing repeats of the reproducible sequence; and labelling the repeats of the reproducible sequence by a detection probe. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification