ACIDOPHILIC FUSARIUM OXYSPORUM STRAINS, METHODS OF THEIR PRODUCTION AND METHODS OF THEIR USE

• US 20180100171A1
• Filed: 10/23/2017
• Published: 04/12/2018
• Est. Priority Date: 07/03/2014
• Status: Active Grant

• Current Assignee: Fynder Group Incorporated
• Original Assignee: Sustainable Products, LLC
• Inventors: KOZUBAL, Mark A., MACUR, Richard E., INSKEEP, William P.
• Granted Patent: US 10,344,306 B2
• Time in Patent Office: Days
• CPC Class Codes: C12N 1/14 Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/145 Fungal isolates
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
• CPC Class Codes: C12P 3/00 Preparation of elements or inorganic compounds except carbon dioxide recovery of carbon dioxides as by-products C12F3/02
• CPC Class Codes: C12P 7/065 with microorganisms other than yeasts
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/6458 by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
• CPC Class Codes: C12P 7/6463 obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/649 Biodiesel, i.e. fatty acid alkyl esters
• CPC Class Codes: C12R 2001/77 Fusarium
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention provides isolated acidophilic Fusarium oxysporum strains, such as MK7, and their progeny, compositions comprising such strains and their progeny, methods of producing such strains and their progeny, and methods of using such strains and their progeny.

Claims

1-11. -11. (canceled)

12. A method of producing one or more energy-rich metabolites, said method comprising the steps of:

a) making a mixture by contacting an isolated Fusarium oxysporum strain MK7 (ATCC Accession Deposit No. PTA-10698), and/or its progeny, with a feedstock material selected from the group consisting of a ligno cellulosic feedstock, a carbon containing waste product, a carbohydrate, and a combination thereof, wherein the feedstock material can support the growth of said Fusarium oxysporium strain MK7 and/or its progeny;

b) incubating said mixture at pH <

0.5-8.0 for a period of time under anaerobic conditions;

c) producing from said mixture one or more energy-rich metabolites following such contact; and

d) optionally, isolating said one or more energy-rich metabolites from the mixture.

13. The method of claim 12, wherein the lignocellulosic feedstock material is wheat straw.

14. The method of claim 12, wherein the energy-rich metabolite is ethanol.

15. The method of claim 12, wherein the feedstock is a lignocellulosic feedstock and the energy-rich metabolite is hydrogen gas.

20. The method of claim 12, further comprising a pretreatment step, wherein said pretreatment step is selected from the group consisting of:

reducing the pH of the mixture, adding manganese to the mixture and adding a nutrient to the mixture.

21. The method according to claim 20, wherein the pH is <

0.5-<

3.0 and the manganese concentration is 5 mM, 10 mM, or 25 mM.

22. The method according to claim 12, wherein the pH is 0.5-3.0.

23. The method according to claim 12, wherein the pH is 2.0-7.0.

24. The method according to claim 12, wherein the pH is 3-4.5.

25. The method according to claim 12, wherein the energy-rich metabolite is a biofuel.

26. The method according to claim 12, wherein the energy-rich metabolite is a biofuel precursor selected from the group consisting of isobutanol, isopropanol, propanol, 2-butanol, 30 butanol, pentanol, 2-pentanol, 3-pentanol, 3-methyl-1-butanol, 2-methyl-1-butanol, and 3-methyl-2-butanol.

27. The method according to claim 12, wherein the carbon containing waste product is municipal, industrial, and/or farm sewage sludge.

28. The method according to claim 27, wherein the pH is 0.7-8.0.

29. The method according to claim 12, wherein the carbohydrate is glucose.

30. The method according to claim 12, wherein the energy-rich metabolite is butanol or isobutanol.

31. The method according to claim 20, wherein the pretreatment step is adding manganese to the mixture.

32. The method according to claim 31, wherein the final manganese concentration in the mixture is 5 mM, 10 mM, or 25 mM.

33. The method according to claim 31, wherein the manganese is selected from the group consisting of Mn₂(CO)₁₀, K₅[Mn(CN)₆NO, MnCl₂, MnF₂, MnBr₂, MnO, MnO_2, MnCl₃, MnF₃, MnBr₃, MnCO_3, Mn(CH₃COO)_2, C₆H₉MnO_6, MnTiO₃, [CH₃COCH═

C(O)CH₃]₂Mn, [C₆H₁₁(CH₂)₃CO₂]₂Mn, (HCO₂)₂Mn, Mn(C₅HF₆O₂)_2, Mn(PH₂O₂)2, MnI_2, (C₃H₅O₃)₂Mn, MnMoO_4, Mn(NO₃)_2, Mn(ClO₄)_2, C₃₂H₁₆MnN_8, MnSO_4, (CH₃COO)₃Mn, C₃₂H₁₆ClMnN_8, C₄₈H₂₈ClMnN₄O_8, C₅H₄CH₃Mn(CO)_3, Mn(C₅H₄C₂H₅)_2, and C₁₆H₂₂Mn.

16-19. -19. (canceled)

34. A method of producing one or more energy-rich metabolites, said method comprising the steps of:

(a) making a mixture by contacting an isolated Fusarium oxysporum strain MK7 (ATCC Accession Deposit No. PTA-10698), and/or its progeny, with a wheat straw feedstock material that supports the growth of the Fusarium oxysporum strain MK7 and/or its progeny;

(b) adding manganese to the mixture to a final concentration of 5-25 mM;

(c) incubating said mixture at pH <

0.5-8.0 for a period of time under anaerobic conditions to produce fungal biomass;

(d) producing from the mixture and/or fungal biomass one or more energy-rich metabolites following such contact, wherein production of the one or more energy-rich metabolites is improved compared to a control lacking manganese; and

(e) optionally, isolating said one or more energy-rich metabolites from the mixture.

35. The method of claim 34, wherein the pH is less than 3.0.

36. A method of producing one or more energy-rich metabolites, said method comprising the steps of:

(a) making a mixture by contacting an isolated Fusarium oxysporum strain MK7 (ATCC Accession Deposit No. PTA-10698), and/or its progeny, with a wheat straw feedstock material that supports the growth of the Fusarium oxysporum strain MK7 and/or its progeny;

(b) adding manganese peroxide isolated from Fusarium oxysporum strain MK7 (ATCC Accession Deposit No. PTA-10698) to the mixture;

(c) incubating said mixture at a pH of less than 3.0 for a period of time under anaerobic conditions to produce fungal biomass;

(d) producing from the mixture and/or fungal biomass one or more energy-rich metabolites following such contact, wherein production of the one or more energy-rich metabolites is improved compared to a control lacking manganese peroxidase isolated from Fusarium oxysporum strain MK7; and

(e) optionally, isolating said one or more energy-rich metabolites from the mixture.