Methods and Compositions for Segregating Target Nucleic Acid From Mixed Nucleic Acid Samples
2 Assignments
0 Petitions
Accused Products
Abstract
The invention provides methods, compositions and kits for segregating a target nucleic acid from a mixed nucleic acid sample. The methods, compositions and kits comprise a non-processive endonuclease (e.g., a restriction enzyme) or an antibody that binds the target nucleic acid (e.g., has methylation specificity). The mixed nucleic acid sample can comprise prokaryotic and eukaryotic nucleic acid and/or nucleic acid from more than one prokaryotic or eukaryotic organisms.
-
Citations
63 Claims
-
1-48. -48. (canceled)
-
49. A method for enriching a target nucleic acid in a mixed sample containing the target nucleic acid and a non-target nucleic acid, comprising:
-
(i) digesting the mixed sample with a methylation-sensitive or methylation-dependent endonuclease that cleaves the target nucleic acid and not the non-target nucleic acid; (ii) digesting the sample with an endonuclease;
wherein the endonuclease cleaves the non-target nucleic acid and not the target nucleic acid, resulting in non-target nucleic acid ends that are incompatible with the cleaved target nucleic acid;(iii) ligating a linker to the cleaved target nucleic acid, or circularizing the cleaved target nucleic acid; and (iv) depleting the non-target nucleic acid. - View Dependent Claims (50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62)
-
-
63-80. -80. (canceled)
Specification