METHOD AND CULTURE DEVICE FOR DETECTING YEASTS AND MOLDS

• US 20180148675A1
• Filed: 01/25/2018
• Published: 05/31/2018
• Est. Priority Date: 02/04/2013
• Status: Active Grant

• Current Assignee: Neogen Food Safety US Holdco Corporation (Neogen Corporation)
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: CHANDRAPATI, SAILAJA, NORDBY, TERA M.
• Granted Patent: US 10,640,743 B2
• Time in Patent Office: Days
• CPC Class Codes: C12M 23/04 Flat or tray type, drawers C12M23/10, C12M23/12, C12M23/16 take precedence
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12M 23/24 Gas permeable parts
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72

Abstract

A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.

Claims

1. A culture device, comprising:

a body comprising a self-supporting substrate having a first major surface and a second major surface;

a first adhesive composition disposed on a portion of the first major surface of the substrate;

a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition;

a cover sheet attached to the body member, wherein the cover sheet comprises a first major surface facing the body member;

a second adhesive composition disposed on a portion of the first major surface of the cover sheet;

a substantially dry, cold-water-soluble second hydrogel-forming composition adhered to the second adhesive composition; and

a plurality of indicator agents, the plurality of indicator agents comprising;

three indicator agents for detecting distinct glycosidase enzyme activities;

an indicator agent for detecting an alkyl esterase enzyme activity;

an indicator agent for detecting a phosphatase enzyme activity;

wherein each of the plurality of indicator agents comprises a detectable reporter group;

wherein at least one of the plurality of indicator agents is disposed in the first adhesive composition, the second adhesive composition, the first hydrogel-forming composition, and/or the second hydrogel-forming composition.

2-20. -20. (canceled)