MODULATED NUTRITIONAL QUALITY TRAITS IN SEEDS

• US 20180153174A1
• Filed: 12/01/2017
• Published: 06/07/2018
• Est. Priority Date: 12/01/2016
• Status: Active Grant

• Current Assignee: Indigo Ag, Inc.
• Original Assignee: Indigo Ag, Inc.
• Inventors: Riley, Raymond, Djonovic, Slavica, Vosnidou, Nancy, Bitas, Vasileios
• Granted Patent: US 10,624,351 B2
• Time in Patent Office: Days
• CPC Class Codes: A01H 17/00 Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza lichens A01H15/00
• CPC Class Codes: A01H 3/00 Processes for modifying phenotypes , e.g. symbiosis with bacteria A01H4/00 takes precedence
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: A01N 63/30 Microbial fungi; Substances produced thereby or obtained therefrom

Abstract

This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte.

Claims

1. A method of modulating the composition of a seed produced by a soybean plant, comprising heterologously disposing an endophyte to a soybean plant element in an amount effective to alter the composition of the seed produced by the soybean plant element relative to seed produced by a reference soybean plant element not further comprising the endophyte.

2. The method of claim 1, wherein the seed produced by the plant element heterologously disposed with the endophyte exhibits an increase in fat composition as compared to the seed produced by the reference soybean plant element not further comprising the endophyte.

3. The method of claim 1, wherein the endophyte comprises a polynucleotide sequence that is at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs. 16, 17, 26, 27, 28, 29, 30, 31, 32 and 33.

4. The method of claim 1, wherein the endophyte is a Curvularia spicifera as deposited under NRRL Culture Deposit No. NRRL-67467, or a modified endophyte derived from the deposit that retains the ability to increase fat composition of a seed produced by a plant element heterologously disposed with the endophyte.

5. The method of claim 1, wherein the seed produced by the plant element heterologously disposed with the endophyte exhibits an increase in carbohydrate composition as compared to the seed produced by the reference soybean plant element not further comprising the endophyte.

6. The method of claim 1, wherein the endophyte comprises a polynucleotide sequence that is at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs. 20, 21, 24, and 25.

7. An animal feed derived from the modulated soybean seed produced by the method of claim 1.

8. A soybean for oil extraction comprising the modulated soybean seed produced by the method of claim 1.

9. A human food product derived from the modulated soybean seed produced by the method of claim 1.

10. An industrial product derived from the modulated soybean seed produced by the method of claim 1.

11-36. -36. (canceled)

37. A synthetic composition, comprising a soybean plant element and an endophyte heterologously disposed to the plant element, wherein the endophyte is capable of altering the composition of seed produced by the plant element relative to seed produced by a reference soybean plant element not further comprising the endophyte.

38. The synthetic composition of claim 37, wherein the seed produced from the plant element heterologously disposed with the endophyte exhibits an increase in fat composition as compared to the seed produced by the reference plant element not further comprising the endophyte.

39. The synthetic composition of claim 37, wherein the endophyte comprises a polynucleotide sequence that is at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs. 16, 17, 26, 27, 28, 29, 30, 31, 32 and 33.

40. The synthetic composition of claim 37, wherein the endophyte is a Curvularia spicifera as deposited under NRRL Culture Deposit No. NRRL-67467, or a modified endophyte derived from the deposit that retains the ability to increase fat composition of a seed produced by a plant element heterologously disposed with the endophyte.

41. The synthetic composition of claim 37, wherein the seed exhibits an increase in carbohydrate composition as compared to the seed produced by the reference soybean plant element not further comprising the endophyte.

42. The synthetic composition of claim 37, wherein the endophyte comprises a polynucleotide sequence that is at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs. 20, 21, 24, and 25.

43. An animal feed derived from the soybean seed produced by the plant element heterologously disposed with the endophyte of claim 37.

44. A soybean for oil extraction comprising the modulated soybean seed produced by the plant element heterologously disposed with the endophyte of claim 37.

45. A human food product derived from the modulated soybean seed produced by the plant element heterologously disposed with the endophyte of claim 37.

46. An industrial product derived from the modulated soybean seed produced by the plant element heterologously disposed with the endophyte of claim 37.

47-72. -72. (canceled)