METHOD FOR STERILIZING MEMBRANE COMPRISING GLUCOSE OXIDASE AND ASSOCIATED BIO-SENSOR

• US 20180154026A1
• Filed: 01/30/2018
• Published: 06/07/2018
• Est. Priority Date: 05/31/2012
• Status: Active Grant

• Current Assignee: Global Life Sciences Solutions USA LLC (Danaher Corporation)
• Original Assignee: General Electric Company
• Inventors: Xu, Yida, Chen, Rui, Chen, Lin
• Granted Patent: US 10,369,238 B2
• Time in Patent Office: Days
• CPC Class Codes: A61L 2/0035 Gamma radiation
• CPC Class Codes: A61L 2/081 Gamma radiation
• CPC Class Codes: C12M 41/32 of substances in solution
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/26 involving oxidoreductase

Abstract

A method for sterilizing a membrane comprising an oxidoreductase enzyme, comprises: irradiating with gamma radiation the membrane comprising an oxidoreductase enzyme soaked in an aqueous buffer solution. Associated biosensors and bioreactors are also described.

Claims

1. A method for sterilizing a membrane comprising an oxidoreductase enzyme, comprising:

irradiating with gamma radiation the membrane comprising an oxidoreductase enzyme soaked in an aqueous buffer solution.

2. The method of claim 1, wherein the oxidoreductase enzyme is selected from the group consisting of glucose oxidase, lactate oxidase and glutamate oxidase.

3. The method of claim 2, wherein the oxidoreductase enzyme is glucose oxidase.

4. The method of claim 1, wherein the aqueous buffer solution is a phosphate buffer.

5. The method of claim 4, wherein the aqueous buffer solution is a phosphate buffer saline (PBS) solution.

6. The method of claim 1, wherein the aqueous buffer solution is substantially free from organic components.

8. The method of claim 1, wherein a dose of the gamma radiation is in a range of from about 25 kGy to about 40 kGy.

9. The method of claim 1, wherein a rate of the gamma radiation is about 20 kGy/hour.

10. The method of claim 1, wherein the membrane is in a plastic container.

11. The method of claim 1, wherein the membrane is in a biosensor.

12. The method of claim 11, wherein the biosensor comprises a Pt electrode and an Ag/AgCl electrode.

13. A biosensor comprising the membrane sterilized using the method of claim 1.

14. The biosensor of claim 13, wherein the membrane comprises glucose oxidase and the sensitivity of the biosensor to glucose is about 80% of the sensitivity of a biosensor comprising a membrane comprising glucose oxidase without sterilization to glucose.

15. The biosensor of claim 13, comprising a Pt electrode and an Ag/AgCl electrode.

17. A bioreactor comprising the biosensor of claim 13.

7. A method for sterilizing a membrane comprising glucose oxidase, comprising:

irradiating with gamma radiation the membrane comprising glucose oxidase soaked in a phosphate buffer saline (PBS) solution.

16. A biosensor comprising a membrane comprising glucose oxidase, sterilized by:

irradiating with gamma radiation while the membrane comprising glucose oxidase is soaked in a phosphate buffer saline (PBS) solution.