METHOD AND APPARATUS FOR RAPID SUB-DIFFRACTION INFRARED IMAGING AND SPECTROSCOPY AND COMPLEMENTARY TECHNIQUES
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Accused Products
Abstract
Methods and apparatus for performing chemical spectroscopy on samples from the scale of nanometers to millimeters or more with a multifunctional platform combining analytical and imaging techniques including atomic force microscopy, infrared spectroscopy, confocal microscopy, Raman spectroscopy and mass spectrometry. For infrared spectroscopy, a sample is illuminated with infrared light and the resulting sample distortion is read out with either a focused UV/visible light beam and/or AFM tip. Using the AFM tip or the UV/visible light beam it is possible to measure the IR absorption characteristics of a sample with spatial resolution ranging from around 1 μm or less to the nanometer scale. The combination of both techniques provides a rapid and large area survey scan with the UV/visible light and a high resolution measurement with the AFM tip. The methods and apparatus also include the ability to analyze light reflected/scattered from the sample via a Raman spectrometer for complementary analysis by Raman spectroscopy. Using a UV/vis source or IR source at higher intensity it is possible to thermally desorb material from a sample for analysis by mass spectrometry. The AFM tip can also be heated to desorb material for mass spec analysis at even higher spatial resolution.
21 Citations
64 Claims
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1-21. -21. (canceled)
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22. An apparatus for rapidly characterizing a sample with infrared radiation on a submicron scale, the apparatus comprising:
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a source of infrared radiation configured to illuminate a sample with a beam of infrared radiation to create an infrared illuminated area; a source of ultraviolet-visible (UV-vis) radiation configured to illuminate at least a region of the infrared illuminated area of the sample with a beam of UV-vis light; a collector configured to collect as collected light at least a portion of the UV-vis light that is at least one of scattered, refracted, and reflected from the sample; a receiver configured to analyze the collected light as indicative of an infrared absorption of the region of the infrared illuminated area; a scanner configured to create relative motion between the sample and at least one of the beam of infrared light and the beam of UV-vis light to measure the infrared absorption of the sample over a scan area that includes a plurality of locations on the sample; and an atomic force microscope subsystem configured to measure a response of a cantilever probe over at least a portion of the scan area. - View Dependent Claims (23, 24, 25)
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26. An apparatus for rapidly characterizing a sample with infrared radiation on a submicron scale, the apparatus comprising:
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a source of infrared radiation configured to illuminate a sample with a beam of infrared radiation to create an infrared illuminated area; a source of ultraviolet-visible (UV-vis) radiation configured to illuminate at least a region of the infrared illuminated area of the sample with a beam of UV-vis light; a collector configured to collect as collected light at least a portion of the UV-vis light that is at least one of scattered, reflected and refracted from the sample; a receiver configured to analyze the collected light as indicative of an infrared absorption of the region of the infrared illuminated area; and a Raman spectrometer configured to analyze the collected light to analyze a Raman response of the sample. - View Dependent Claims (27, 28, 29)
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30. An apparatus for rapidly characterizing a sample with infrared radiation on a submicron scale, the apparatus comprising:
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a source of infrared radiation configured to illuminate a sample with a beam of infrared radiation to create an infrared illuminated area; a source of ultraviolet-visible (UV-vis) radiation configured to illuminate at least a region of the infrared illuminated area of the sample with a beam of UV-vis light; a collector configured to collect as collected light at least a portion of the UV-vis light that is at least one of scattered, reflected and refracted from the sample; and a receiver configured for confocal optical microscopy and further configured to analyze the collected light as indicative of infrared absorption of the region of the infrared illuminated area of the sample. - View Dependent Claims (31)
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32. An apparatus for rapidly characterizing a sample with infrared radiation on a submicron scale, the apparatus comprising:
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a broadband source of infrared radiation configured to illuminate a sample with a beam of infrared radiation to create an infrared illuminated area; a source of ultraviolet-visible (UV-vis) radiation configured to illuminate at least a region of the infrared illuminated area of the sample with a beam of UV-vis light; a collector configured to collect as collected light at least a portion of the UV-vis light that is at least one of scattered, reflected and refracted from the sample; and a receiver configured to analyze the collected light as indicative of infrared absorption of the region. - View Dependent Claims (33, 34, 35)
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36. An apparatus for rapidly characterizing a sample with infrared radiation on a submicron scale, the apparatus comprising:
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a source of infrared radiation configured to illuminate a sample with a beam of infrared radiation to create an infrared illuminated area; a source of ultraviolet-visible (UV-vis) radiation configured to illuminate at least a region of the infrared illuminated area of the sample with a beam of UV-vis light; a collector configured to collect as collected light at least a portion of the UV-vis light that is at least one of scattered, reflected and refracted from the sample; and a receiver configured to analyze the collected light as indicative of an infrared absorption of the sample; wherein the receiver comprises at least one of a position sensitive detector and an array detector; and wherein the infrared absorption of the sample is measured with a spatial resolution of less than or equal to 1 micrometer.
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37. An apparatus for rapidly characterizing a sample with infrared radiation on a submicron scale, the apparatus comprising:
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a source of infrared radiation configured to illuminate a sample with a beam of infrared radiation to create an infrared illuminated area; a source of ultraviolet-visible (UV-vis) radiation configured to illuminate at least a region of the infrared illuminated area of the sample with a beam of UV-vis light; a collector configured to collect as collected light at least a portion of the UV-vis light that is at least one of scattered, reflected and refracted from the sample; and a receiver configured to analyze the collected light as indicative of an infrared absorption of the sample; wherein at least one of the collector and the receiver includes a filter to block at least a portion of the UV-vis light. - View Dependent Claims (38)
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39. An apparatus for rapidly characterizing a sample with infrared radiation on a submicron scale, the apparatus comprising:
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a source of infrared radiation configured to illuminate a sample with a beam of infrared radiation; a first focusing optic configured to focus the beam of infrared radiation to form an infrared illuminated region of a sample; a source of ultraviolet-visible (UV-vis) radiation configured to illuminate a sub-region of the sample with a beam of UV-vis light; a second focusing optic configured to focus the beam of UV-vis light at the sub-region of the sample, wherein the sub-region at least partially overlaps the infrared illuminated region; a collector configured to collect as collected light at least a portion of the UV-vis light that is at least one of scattered, reflected and refracted from interaction of the beam of UV-vis light with the sample; a receiver configured to analyze the collected light and provide an indication of an infrared absorption of the sub-region. - View Dependent Claims (40, 41, 42, 43, 44)
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45. A method for rapidly characterizing a sample with infrared radiation on a submicron scale, the method comprising:
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illuminating a sample with a beam of infrared radiation to create an infrared illuminated area; illuminating at least a region of the infrared illuminated area of the sample with a beam of ultraviolet-visible (UV-vis) light; collecting as collected light at least a portion of the UV-vis light that is at least one of scattered, refracted, and reflected from the sample; analyzing the collected light to determine an infrared absorption of the region of the infrared illuminated area; creating relative motion between the sample and at least one of the beam of infrared light and the beam of UV-vis light to measure the infrared absorption of the sample over a scan area that includes a plurality of locations on the sample; and measuring a response of a cantilever probe over at least a portion of the scan area with an atomic force microscope. - View Dependent Claims (46, 47, 48)
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49. A method for rapidly characterizing a sample with infrared radiation on a submicron scale, the method comprising:
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illuminating a sample with a beam of infrared radiation to create an infrared illuminated area; illuminating at least a region of the infrared illuminated area of the sample with a beam of ultraviolet-visible (UV-vis); collecting as collected light at least a portion of the UV-vis light that is at least one of scattered, refracted, and reflected from the sample; analyzing the collected light to determine an infrared absorption of the region of the infrared illuminated area; and analyzing the collected light to detect a Raman response of the sample. - View Dependent Claims (50, 51, 52)
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53. A method for rapidly characterizing a sample with infrared radiation on a submicron scale, the method comprising:
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illuminating a sample with a beam of infrared radiation to create an infrared illuminated area; illuminating at least a region of the infrared illuminated area of the sample with a beam of ultraviolet-visible (UV-vis) light; collecting as collected light at least a portion of the UV-vis light that is at least one of scattered, refracted, and reflected from the sample; analyzing the collected light at a receiver configured for confocal optical microscopy, wherein analyzing the collected light includes determining an infrared absorption of the region of the infrared illuminated area. - View Dependent Claims (54)
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55. A method for rapidly characterizing a sample with infrared radiation on a submicron scale, the method comprising:
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illuminating a sample with a beam of infrared radiation from a broadband source of infrared radiation to create an infrared illuminated area; illuminating at least a region of the infrared illuminated area of the sample with a beam of ultraviolet-visible (UV-vis) light; collecting as collected light at least a portion of the UV-vis light that is at least one of scattered, refracted, and reflected from the sample; analyzing the collected light to determine an infrared absorption of the region of the infrared illuminated area; and analyzing the collected light to detect a Raman response of the sample. - View Dependent Claims (56, 57, 58)
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59. A method for rapidly characterizing a sample with infrared radiation on a submicron scale, the method comprising:
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illuminating a sample with a beam of infrared radiation from a source of infrared radiation to create an infrared illuminated area; illuminating at least a region of the infrared illuminated area of the sample with a beam of ultraviolet-visible (UV-vis) light; collecting as collected light at least a portion of the UV-vis light that is at least one of scattered, refracted, and reflected from the sample; and analyzing the collected light at a receiver having a position sensitive detector and an array detector to determine an infrared absorption of the region of the infrared illuminated area, wherein the infrared absorption of the infrared illuminated area is measured with a spatial resolution of less than or equal to 1 micrometer.
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60. A method for rapidly characterizing a sample with infrared radiation on a submicron scale, the method comprising:
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illuminating a sample with a beam of infrared radiation to create an infrared illuminated area; illuminating at least a region of the infrared illuminated area of the sample with a beam of ultraviolet-visible (UV-vis) light; collecting as collected light at least a portion of the UV-vis light that is at least one of scattered, refracted, and reflected from the sample; analyzing the collected light at a receiver to determine an infrared absorption of the region of the infrared illuminated area; and blocking at least a portion of the UV-vis light with a filter arranged at at least one of the collector and the receiver. - View Dependent Claims (61)
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62. A method for rapidly characterizing a sample with infrared radiation on a submicron scale, the method comprising:
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illuminating a sample with a beam of infrared radiation; focusing the beam of infrared radiation at an infrared illuminated region of the sample with a first focusing optic; illuminating at least a region of the infrared illuminated area of the sample with a beam of ultraviolet-visible (UV-vis) light; focusing the beam of UV-vis light at a sub-region of the sample that partially overlaps the infrared illuminated region with a second focusing optic; collecting as collected light at least a portion of the UV-vis light that is at least one of scattered, refracted, and reflected from the sample; and analyzing the collected light to determine an infrared absorption of the region of the infrared illuminated area. - View Dependent Claims (63, 64)
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Specification