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SELECTIVE AMPLIFICATION USING BLOCKING OLIGONUCLEOTIDES

  • US 20180216174A1
  • Filed: 01/19/2018
  • Published: 08/02/2018
  • Est. Priority Date: 02/01/2017
  • Status: Active Grant
First Claim
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1. A method of selective amplification, comprising:

  • providing a sample comprising a plurality of nucleic acid target molecules and one or more undesirable nucleic acid species;

    providing a plurality of oligonucleotide probes, wherein each of the plurality of oligonucleotide probes comprises a molecular label sequence and a binding region;

    contacting the plurality of oligonucleotide probes with the plurality of nucleic acid target molecules for hybridization;

    extending oligonucleotide probes that are hybridized to the plurality of nucleic acid target molecules to generate a plurality of extension products;

    providing a blocking oligonucleotide that specifically binds to at least one of the one or more undesirable nucleic acid species; and

    amplifying the plurality of extension products to generate a plurality of amplicons,whereby the amplification or the extension of the undesirable nucleic acid species is reduced by the blocking oligonucleotide.

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