Nucleic Acid Sample Preparation
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Abstract
This invention relates to the preparation of nucleic acid samples for analysis. The invention may be particularly useful for single stranded samples. Embodiments of the invention involve the attachment of double stranded or hairpin oligonucleotides using template independent polymerase enzymes in the preparation of nucleic acid sequencing libraries.
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Citations
42 Claims
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1-24. -24. (canceled)
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25. A method comprising:
- contacting a single stranded oligonucleotide with an enzyme, wherein said enzyme catalyzes an addition of a nucleotide to an end of said single stranded oligonucleotide in a template independent manner, wherein said nucleotide comprises a 5′
-triphosphate. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
- contacting a single stranded oligonucleotide with an enzyme, wherein said enzyme catalyzes an addition of a nucleotide to an end of said single stranded oligonucleotide in a template independent manner, wherein said nucleotide comprises a 5′
Specification