METHODS AND COMPOSITIONS FOR EXPANSION AND DIFFERENTIATION OF SKELETAL MUSCLE STEM CELLS OR PROGENITOR CELLS
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Abstract
The present disclosure describes compositions for preparing a myofiber or myotube from a skeletal muscle stem cell or progenitor cell comprising a carnitine and/or a derivative thereof, a fatty acid a steroid and combinations thereof. Preferred embodiment comprises of 0.1 mM L-carnitine, 0.2 mM 9-cis-linoleic acid and 10 mM dihydrotestosterone. Also disclosed is a composition for inducing expansion of skeletal muscle stem cells or progenitor cells comprising a fibroblast growth factor agonist, a Notch signalling agonist, a nucleic acid, and combinations thereof. Preferred embodiment comprises 20 ng/ml basic fibroblast growth factor (bFGF), 50 μg/ml Delta-like ligand 1 (DLL1), 10 mM hypoxanthine and 1.6 mM thymidine.
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59 Claims
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1-38. -38. (canceled)
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39. :
- A composition for preparing a myofiber or myotube from a skeletal muscle stem cell or progenitor cell comprising a carnitine and/or a derivative thereof, a fatty add and a steroid.
- View Dependent Claims (40, 41, 42, 43, 44, 45, 46, 47, 48)
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49. :
- A composition for inducing expansion of skeletal muscle stem cells or progenitor cells comprising a fibroblast growth factor signalling agonist, a Notch signalling agonist and a nucleic add.
- View Dependent Claims (50, 51, 52, 53, 54, 55, 56, 57)
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58. :
- A method for preparing myofibers or myotubes, comprising contacting a skeletal muscle stem cell or progenitor cell with a composition for preparing a myofiber or myotube from a skeletal muscle stem cell or progenitor cell comprising a carnitine and/or a derivative thereof, a fatty add and a steroid.
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59. :
- A method for inducing expansion of skeletal muscle progenitor cells comprising contacting a skeletal muscle stem cell or progenitor cell with a composition for inducing expansion of skeletal muscle stem cells or progenitor cells comprising a fibroblast growth factor signalling agonist, a Notch signalling agonist and a nucleic add.
Specification