MULTIPLE HYBRID IMMUNOASSAY
First Claim
Patent Images
1. A cartridge for sensing at least one analyte in a sample, said cartridge comprising:
- a sample holding chamber for receiving said sample and retaining said sample;
a first conduit connected to said sample holding chamber;
at least one analyte sensor, wherein said sensor comprises an analyte-responsive surface and said surface is within said first conduit;
a second conduit for retaining a fluid, said second conduit connected to said first conduit;
a valve connected to an opening in said first conduit, wherein said valve is closed by contact with said sample;
a pump capable of displacing said sample from said holding chamber into said first conduit, wherein said pump, said valve, and said first and second conduits are configured such that said fluid from said second conduit can be displaced into said first conduit upon closure of the valve; and
an immunoassay composition for detecting said analyte, said immunoassay composition comprising at least three different antibodies, the at least three different antibodies being capable of binding to at least three different epitopes on said analyte, in which at least two of the at least three different antibodies are capable of binding to at least two different epitopes on a subform of the analyte, and in which at least one of the at least three different epitopes on the analyte is unavailable for binding on the subform.
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Accused Products
Abstract
The invention relates to compositions and methods for the immunoassay of an analyte of interest. The analyte is detected in an immunoassay using three or more antibodies, where in each antibody specifically binds to a different epitope on the analyte. When the analyte of interest in a clinical marker for an acute disease, the detection of the analyte by immunoassay is a diagnosis of the occurrence of the disease.
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Citations
30 Claims
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1. A cartridge for sensing at least one analyte in a sample, said cartridge comprising:
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a sample holding chamber for receiving said sample and retaining said sample; a first conduit connected to said sample holding chamber; at least one analyte sensor, wherein said sensor comprises an analyte-responsive surface and said surface is within said first conduit; a second conduit for retaining a fluid, said second conduit connected to said first conduit; a valve connected to an opening in said first conduit, wherein said valve is closed by contact with said sample; a pump capable of displacing said sample from said holding chamber into said first conduit, wherein said pump, said valve, and said first and second conduits are configured such that said fluid from said second conduit can be displaced into said first conduit upon closure of the valve; and an immunoassay composition for detecting said analyte, said immunoassay composition comprising at least three different antibodies, the at least three different antibodies being capable of binding to at least three different epitopes on said analyte, in which at least two of the at least three different antibodies are capable of binding to at least two different epitopes on a subform of the analyte, and in which at least one of the at least three different epitopes on the analyte is unavailable for binding on the subform. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A cartridge for sensing at least one analyte in a sample, said cartridge comprising:
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a sample holding chamber for receiving said sample and retaining said sample; a first conduit connected to said sample holding chamber; at least one analyte sensor, wherein said sensor comprises an analyte-responsive surface and said surface is within said first conduit; a second conduit for retaining a fluid, said second conduit connected to said first conduit; a pump capable of displacing said sample from said holding chamber into said first conduit; a valve connected to an opening in said first conduit, wherein said valve is closed by contact with said sample, and wherein said pump, said valve, and said first and second conduits are configured such that said fluid from said second conduit can be displaced into said first conduit upon closure of the valve; means for inserting at least one air segment into said first or second conduit; and an immunoassay composition for detecting said analyte comprising at least three different antibodies, the at least three different antibodies being capable of binding to at least three different epitopes on an analyte, in which at least two of the at least three different antibodies are capable of binding to at least two different epitopes on a subform of the analyte, and in which at least one of the at least three different epitopes on the analyte is unavailable for binding on the subform. - View Dependent Claims (24, 25)
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26. A cartridge for sensing at least one analyte in a sample, said cartridge comprising:
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a sample holding chamber for receiving said sample and retaining said sample; a first conduit connected to said sample holding chamber; at least one analyte sensor, wherein said sensor comprises an analyte-responsive surface and said surface is within said first conduit; a second conduit for retaining a fluid, said second conduit connected to said first conduit; a valve connected to an opening in said first conduit, wherein said valve is permanently closed by contact with said sample; a pump capable of displacing said sample from said holding chamber into said first conduit, said pump further capable of displacing said fluid from said second conduit into said first conduit; and an immunoassay composition for detecting said analyte comprising at least three different antibodies, the at least three different antibodies being capable of binding to at least three different epitopes on an analyte, in which at least two of the at least three different antibodies are capable of binding to at least two different epitopes on a subform of the analyte, and in which at least one of the at least three different epitopes on the analyte is unavailable for binding on the subform. - View Dependent Claims (27, 28)
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29. An enzyme conjugate, comprising an enzyme covalently attached to at least two types of Fab fragments wherein each Fab fragment is derived from a different troponin I antibody, and wherein said different antibodies target different epitopes on troponin I.
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30. A method of forming an immobilized troponin I assay capture layer on a sensor surface, comprising:
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(a) forming microparticle beads covalently attached to at least two different troponin I antibodies wherein the at least two antibodies target different epitopes on troponin I, (b) forming a suspension of said microparticle beads; (c) dispensing the suspension onto a sensor surface; and (d) allowing said suspension to dry on said sensor surface to form a layer of immobilized microparticle beads.
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Specification