METHOD FOR SURFACE PLASMON RESONANCE FLUORESCENCE ANALYSIS AND DEVICE FOR SURFACE PLASMON RESONANCE FLUORESCENCE ANALYSIS
First Claim
1. A surface plasmon resonance fluorescence analysis method of detecting presence or an amount of a detection object substance by detecting fluorescence that is emitted by a fluorescence material labelling the detection object substance when the fluorescence material is excited by localized light based on a surface plasmon resonance, the surface plasmon resonance fluorescence analysis method comprising:
- acquiring output information of an excitation light irradiator by applying excitation light from the excitation light irradiator to an analysis chip installed in a chip holder, and by detecting excitation light reflected by the analysis chip, the analysis chip including a prism including an incidence surface and a film formation surface, a metal film disposed on the film formation surface, and a capturing body fixed on the metal film.
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Accused Products
Abstract
The present invention includes a prism having a light incidence surface and a film formation surface, a metal film disposed on the film formation surface, and a trapping body secured to the metal film. Excitation light is irradiated from an excitation light irradiation part onto an analysis chip installed in a chip holder, and excitation light reflected by the analysis chip is detected. The information outputted by the excitation light irradiation part is acquired.
20 Citations
14 Claims
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1. A surface plasmon resonance fluorescence analysis method of detecting presence or an amount of a detection object substance by detecting fluorescence that is emitted by a fluorescence material labelling the detection object substance when the fluorescence material is excited by localized light based on a surface plasmon resonance, the surface plasmon resonance fluorescence analysis method comprising:
acquiring output information of an excitation light irradiator by applying excitation light from the excitation light irradiator to an analysis chip installed in a chip holder, and by detecting excitation light reflected by the analysis chip, the analysis chip including a prism including an incidence surface and a film formation surface, a metal film disposed on the film formation surface, and a capturing body fixed on the metal film. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A surface plasmon resonance fluorescence analysis apparatus that detects presence or an amount of a detection object substance by detecting fluorescence that is emitted by a fluorescence material labelling the detection object substance when the fluorescence material is excited by localized light based on a surface plasmon resonance, the surface plasmon resonance fluorescence analysis apparatus comprising:
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a chip holder for detachably holding an analysis chip including a prism including an incidence surface and a film formation surface, a metal film disposed on the film formation surface, and a capturing body fixed on the metal film; an excitation light irradiator that applies excitation light to the analysis chip held by the chip holder; an excitation light detector that detects excitation light reflected by the analysis chip; a fluorescence detector that detects fluorescence emitted from the fluorescence material labelling the detection object substance captured by the capturing body; and a processor that acquires output information of the excitation light irradiator based on a detection result of the excitation light detector. - View Dependent Claims (11, 12, 13, 14)
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Specification