METHODS AND COMPOSITIONS INVOLVING WHEY PROTEIN ISOLATES
First Claim
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1. A method for fractionating a protein mixture comprising multiple protein species to obtain a protein of interest comprising:
- (a) adjusting the pH of said protein mixture based on the isoelectric point of said protein of interest, thereby rendering a net charge of about zero on said protein of interest,(b) adjusting the conductivity of said protein mixture such that said multiple species other than said protein of interest are rejected by a charged ultrafiltration membrane; and
(c) contacting said mixture with said charged ultrafiltration membrane to achieve a first permeate and a first retentate, wherein said ultrafiltration membrane has a pore size at least 100 kDa above, or 10×
greater than, at least one of said multiple species other than said protein of interest, wherein said first permeate comprises an increased ratio of said protein of interest as compared to said protein mixture.
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Abstract
The present invention concerns methods of isolating milk proteins. Methods of the invention include charged ultrafiltration processes that use variations in pH to further separate protein species.
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19 Claims
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1. A method for fractionating a protein mixture comprising multiple protein species to obtain a protein of interest comprising:
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(a) adjusting the pH of said protein mixture based on the isoelectric point of said protein of interest, thereby rendering a net charge of about zero on said protein of interest, (b) adjusting the conductivity of said protein mixture such that said multiple species other than said protein of interest are rejected by a charged ultrafiltration membrane; and (c) contacting said mixture with said charged ultrafiltration membrane to achieve a first permeate and a first retentate, wherein said ultrafiltration membrane has a pore size at least 100 kDa above, or 10×
greater than, at least one of said multiple species other than said protein of interest, wherein said first permeate comprises an increased ratio of said protein of interest as compared to said protein mixture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification