SAMPLE INDEXING FOR SINGLE CELLS
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Accused Products
Abstract
Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.
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Citations
86 Claims
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5. A method for sample identification, comprising:
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contacting one or more cells from each of a plurality of samples with a sample indexing composition of a plurality of sample indexing compositions, wherein each of the one or more cells comprises one or more cellular component targets, wherein at least one sample indexing composition of the plurality of sample indexing compositions comprises a cellular component binding reagent, wherein the cellular component binding reagent is associated with a sample indexing oligonucleotide, wherein at least one of the two or more the cellular component binding reagent is capable of specifically binding to at least one of the one or more cellular component targets, wherein the sample indexing oligonucleotide comprises a sample indexing sequence, and wherein sample indexing sequences of at least two sample indexing compositions of the plurality of sample indexing compositions comprise different sequences; and identifying sample origin of at least one cell of the one or more cells based on the sample indexing sequence of at least one sample indexing oligonucleotide of the plurality of sample indexing compositions. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 14, 18, 19, 23, 24, 30, 32, 44, 48, 49, 50, 57, 65)
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70. A plurality of sample indexing compositions,
wherein each of the plurality of sample indexing compositions comprises a cellular component binding reagent, wherein the cellular component binding reagent is associated with a sample indexing oligonucleotide, wherein the cellular component binding reagent is capable of specifically binding to at least one cellular component target, wherein the sample indexing oligonucleotide comprises a sample indexing sequence for identifying sample origin of one or more cells of a sample, and wherein sample indexing sequences of at least two sample indexing compositions of the plurality of sample indexing compositions comprise different sequences.
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Specification