METHODS FOR REMOVING CYTOKINES FROM BLOOD WITH SURFACE IMMOBILIZED POLYSACCHARIDES
First Claim
1. A device for removing an adsorbate selected from cytokines, pathogens and toxins from blood, the device comprising:
- a container which comprises a solid substrate of high surface area having at least one polysaccharide adsorbent on the surface thereof with a binding affinity for the adsorbate, wherein the at least one polysaccharide comprises a carbohydrate with mannose sequences; and
wherein the solid substrate has interstitial channel spaces large enough to permit the transport of blood cells, and during blood flow the adsorbate binds to binding sites on the at least one polysaccharide adsorbent becoming separated from the blood and the transport of the adsorbates from blood to adsorbent sites on the solid substrate is primarily by convection transport.
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Abstract
The present invention is directed to a method for removing cytokines and/or pathogens from blood or blood serum (blood) by contacting the blood with a solid, essentially non micro-porous substrate which has been surface treated with heparin, heparan sulfate and/or other molecules or chemical groups (the adsorbent media or media) having a binding affinity for the cytokine or pathogen(s) to be removed (the adsorbates), and wherein the size of the interstitial channels within said media is balanced with the amount of media surface area and the surface concentration of binding sites on the media in order to provide adequate adsorptive capacity while also allowing relatively high flow rates of blood through the adsorbent media.
11 Citations
20 Claims
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1. A device for removing an adsorbate selected from cytokines, pathogens and toxins from blood, the device comprising:
- a container which comprises a solid substrate of high surface area having at least one polysaccharide adsorbent on the surface thereof with a binding affinity for the adsorbate, wherein the at least one polysaccharide comprises a carbohydrate with mannose sequences; and
wherein the solid substrate has interstitial channel spaces large enough to permit the transport of blood cells, and during blood flow the adsorbate binds to binding sites on the at least one polysaccharide adsorbent becoming separated from the blood and the transport of the adsorbates from blood to adsorbent sites on the solid substrate is primarily by convection transport. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- a container which comprises a solid substrate of high surface area having at least one polysaccharide adsorbent on the surface thereof with a binding affinity for the adsorbate, wherein the at least one polysaccharide comprises a carbohydrate with mannose sequences; and
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14. A method for removing an adsorbate selected from cytokines, pathogens and toxins from blood, the method comprising:
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contacting blood with a device having a solid substrate of high surface area with at least one polysaccharide adsorbent on the surface thereof with a binding affinity for the adsorbate, wherein the at least one polysaccharide adsorbent comprises a carbohydrate with mannose sequences; and wherein the solid substrate has interstitial channel spaces large enough to permit the transport of blood cells, and during blood flow the adsorbate binds to binding sites on the at least one polysaccharide adsorbent, becoming separated from the blood and wherein the transport of the adsorbates from blood to adsorbent sites on the solid substrate is primarily by convection transport, to thereby remove the adsorbate. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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Specification