METHOD FOR PREPARING COMPOSITION FOR PROMOTING HAIR GROWTH USING NANOG-INTRODUCED MESENCHYMAL STEM CELLS DERIVED FROM FETUS IN AMNIOTIC FLUID

• US 20180362606A1
• Filed: 09/09/2016
• Published: 12/20/2018
• Est. Priority Date: 09/15/2015
• Status: Active Grant

• Current Assignee: STEM Lab, Inc.
• Original Assignee: STEM Lab, Inc.
• Inventors: YOU, Seung Kwon, JUN, Eun Kyoung, PARK, Jung Hyun, YUN, Won-Jin, SON, Da-Ryeon
• Granted Patent: US 10,640,541 B2
• Time in Patent Office: Days
• CPC Class Codes: A61K 35/50 Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
• CPC Class Codes: A61K 38/1709 from mammals
• CPC Class Codes: A61K 38/1825 Fibroblast growth factor [FGF]
• CPC Class Codes: A61K 38/1858 Platelet-derived growth factor [PDGF]
• CPC Class Codes: A61K 38/30 Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2 insulin-like growth factor binding protein A61K38/1754
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/98 of animal origin
• CPC Class Codes: A61P 17/14 for baldness or alopecia
• CPC Class Codes: A61Q 7/00 Preparations for affecting hair growth
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/475 Growth factors; Growth regulators
• CPC Class Codes: C07K 14/49 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C07K 14/50 Fibroblast growth factor [FGF]
• CPC Class Codes: C07K 14/503 basic FGF [bFGF]
• CPC Class Codes: C07K 14/65 Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2

Abstract

The present invention relates to a culture solution of MSCs derived from a fetus in amniotic fluid, and more specifically, to a composition for promoting hair growth or preventing hair loss, which includes a culture solution of MSCs overexpressing a reprogramming factor Nanog and derived from a fetus in amniotic fluid as an active ingredient. In addition, the present invention relates to a method for preparing the composition, which includes culturing Nanog-introduced MSCs derived from a fetus in amniotic fluid in a conditioned medium and collecting the culture solution. The conditioned medium composition according to the present invention exhibits a hair growth promoting effect, and thus is able to be used as cosmetic and pharmaceutical compositions for promoting hair growth.

Claims

1. A method for producing any one or more human growth factors selected from the group consisting of a basic fibroblast growth factor (bFGF), an insulin-like growth factor (IGF), wingless-type MMTV integration site family member 7A (Wnt7a) and a platelet-derived growth factor (PDGF-AA) from Nanog-overexpressing mesenchymal stem cells derived from a fetus in amniotic fluid, the method comprising:

(a) isolating fetal cells from the amniotic fluid obtained from a pregnant woman;

(b) obtaining mesenchymal stem cells derived from a fetus in amniotic fluid by subculturing the isolated fetal cells in a culture medium containing fetal bovine serum (FBS), bFGF, selenium and ascorbic acid;

(c) obtaining Nanog-overexpres sing mesenchymal stem cells derived from a fetus in amniotic fluid by introducing Nanog into the obtained mesenchymal stem cells derived from a fetus in amniotic fluid; and

(d) preparing a conditioned medium containing any one or more human growth factors selected from the group consisting of bFGF, IGF, Wnt7a and PDGF-AA by culturing the obtained Nanog-overexpressing mesenchymal stem cells derived from a fetus in amniotic fluid in a serum-free medium for 1 to 5 days.

2. The method of claim 1, wherein, in Step (c), Nanog is introduced using a retrovirus vector.

3. A method for preparing a composition for promoting hair growth, which comprises a conditioned medium containing any one or more human growth factors selected from the group consisting of a basic fibroblast growth factor (bFGF), an insulin-like growth factor (IGF), wingless-type MMTV integration site family member 7A (Wnt7a) and a platelet-derived growth factor (PDGF-AA) from Nanog-overexpressing mesenchymal stem cells derived from a fetus in amniotic fluid as an active ingredient, the method comprising:

(a) isolating fetal cells from the amniotic fluid obtained from a pregnant woman;

(b) obtaining mesenchymal stem cells derived from a fetus in amniotic fluid by subculturing the isolated fetal cells in a culture medium containing fetal bovine serum (FBS), bFGF, selenium and ascorbic acid;

(c) obtaining Nanog-overexpres sing mesenchymal stem cells derived from a fetus in the amniotic fluid by introducing Nanog into the obtained mesenchymal stem cells derived from a fetus in amniotic fluid;

(d) preparing a conditioned medium containing any one or more human growth factors selected from the group consisting of bFGF, IGF, Wnt7a and PDGF-AA by culturing the obtained Nanog-overexpressing mesenchymal stem cells derived from a fetus in amniotic fluid in a serum-free medium for 1 to 5 days; and

(e) preparing a composition comprising the prepared conditioned medium as an active ingredient.

4. The method of claim 3, wherein, in Step (c), Nanog is introduced using a retrovirus vector.

5. A composition for promoting hair growth which comprises the conditioned medium containing any one or more human growth factors selected from the group consisting of a basic fibroblast growth factor (bFGF), an insulin-like growth factor (IGF), wingless-type MMTV integration site family member 7A (Wnt7a) and a platelet-derived growth factor (PDGF-AA) from Nanog-overexpres sing mesenchymal stem cells derived from a fetus in amniotic fluid as an active ingredient, which was prepared by the method of claim 3.

6. A cosmetic composition for promoting hair growth, comprising the composition of claim 5.

7. A pharmaceutical composition for promoting hair growth, comprising the composition of claim 5.