COMPOSITIONS COMPRISING BACTERIAL STRAINS

• US 20180369292A1
• Filed: 06/28/2018
• Published: 12/27/2018
• Est. Priority Date: 07/13/2016
• Status: Active Grant

• Current Assignee: CJ Bioscience Incorporated
• Original Assignee: 4D Pharma PLC
• Inventors: BERNALIER-DONADILLE, Annick, CROUZET, Laureen, HABOUZIT, Chloe
• Granted Patent: US 10,610,548 B2
• Time in Patent Office: Days
• CPC Class Codes: A23L 33/135 Bacteria or derivatives thereof, e.g. probiotics
• CPC Class Codes: A61K 2035/11 Medicinal preparations comprising living procariotic cells
• CPC Class Codes: A61K 35/74 Bacteria therapeutic use of a bacterial protein A61K38/00
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: A61K 9/19 lyophilised , i.e. freeze-dried, solutions or dispersions lyophilised products with subsequent particle size reduction A61K9/14; granules or pellets made by lyphilisation A61K9/1682; solid oral dosage forms made by lyophilisation A61K9/2095; lyophilisation additives A61K47/00
• CPC Class Codes: A61K 9/48 Preparations in capsules, e.g. of gelatin, of chocolate A61K9/0004 takes precedence; bite capsules A61K9/0056
• CPC Class Codes: A61P 1/10 Laxatives
• CPC Class Codes: A61P 1/12 Antidiarrhoeals
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention provides compositions comprising one or more bacterial strains for treating preventing diarrhea and/or constipation.

Claims

1. A composition comprising a bacterial strain of the genus Blautia, for use in a method of treating or preventing diarrhea and/or constipation.

2. The composition of claim 1, wherein the diarrhea and/or constipation is associated with IBS and/or an inflammatory disease of the intestine.

3. The composition of claim 2, wherein the inflammatory disease is of the small intestine, colon or rectum.

4. The composition of claim 1, wherein the diarrhea and/or constipation is associated with IBS, Crohn'"'"'s disease or ulcerative colitis.

5. The composition of claim 1, wherein the composition is for use in treating or preventing diarrhea and/or constipation in a subject diagnosed with IBS, Crohn'"'"'s disease or ulcerative colitis.

8. The composition of claim 1, wherein the subject additionally has abdominal pain and/or bloating.

9. The composition of claim 1, wherein the subject has an increased level of hydrogen in their breath relative to a healthy subject.

10. The composition of claim 1, wherein the use further comprises reducing the hydrogen level in the breath of the subject.

11. The composition of claim 1, wherein the use further comprises monitoring the hydrogen level in the subject'"'"'s breath during and/or following the treatment or prevention and thereby assessing the likely effectiveness of treatment or prevention.

15. The composition of claim 1, wherein the bacterial strain is of Blautia hydrogenotrophica.

18. The composition of claim 1, wherein the bacterial strain has a 16s rRNA sequence that is at least 95%, 96%, 97%, 98%, 99%, 99.5% or 99.9% identical to the 16s rRNA sequence of a bacterial strain of Blautia hydrogenotrophica.

19. The composition of claim 15, wherein the bacterial strain has a 16s rRNA sequence that is at least 97%, 98%, 99%, 99.5% or 99.9% identical to SEQ ID NO:

5 or which has the 16s rRNA sequence of SEQ ID NO;

5.

22. The composition of claim 1, wherein the composition comprises a bacterial strain of the species Blautia hydrogenotrophica, for use in a method of treating or preventing diarrhea and/or constipation in a subject diagnosed with IBS.

25. The composition of claim 1, wherein the composition is for oral administration.

26. The composition of claim 1, wherein the composition comprises one or more pharmaceutically acceptable excipients or carriers.

27. The composition of claim 1, wherein the bacterial strain is lyophilised.

28. The composition of claim 1, wherein the bacterial strain is viable.

29. The composition of claim 1, wherein the composition comprises a single strain of the genus Blautia.

6. (canceled)

7. (canceled)

12. (canceled)

13. (canceled)

14. (canceled)

16. (canceled)

17. (canceled)

20. (canceled)

21. (canceled)

23. (canceled)

24. (canceled)

30. (canceled)

31. (canceled)

32. (canceled)

33. A method of treating or preventing diarrhea and/or constipation, comprising administering a composition comprising a bacterial strain of the genus Blautia to a patient in need thereof.

34. A method according to claim 33, wherein the Blautia is Blautia hydrogenotrophica.