YEAST STRAINS WITH REDUCED FATTY ALCOHOL OXIDASE ACTIVITY AND METHOD FOR THE PRODUCTION OF OMEGA-HYDROXY FATTY ACIDS AND DICARBOXYLIC ACIDS
First Claim
Patent Images
1. A genetically engineered Yarrowia lipolytica cell, comprising:
- a. a disrupted fatty alcohol oxidase encoding FAO1 YALI0B14014g gene; and
b. at least one disrupted acyl-CoA oxidase encoding gene selected from the group consisting of;
POX1, POX2, POX3, POX4, POX5, and POX6,wherein the engineered Yarrowia lipolytica cell has reduced fatty alcohol oxidase activity relative to a wild type Yarrowia lipolytica cell.
3 Assignments
0 Petitions
Accused Products
Abstract
The invention relates to various new yeast strains of the type Yarrowia lipolytica as well as relevant methods for the biocatalytic preparation of ω-hydroxy fatty acids or dicarboxylic acids with the aid of these strains, whereby the formation of ω-hydroxy fatty acids or dicarboxylic acids is advantageously increased.
4 Citations
20 Claims
-
1. A genetically engineered Yarrowia lipolytica cell, comprising:
-
a. a disrupted fatty alcohol oxidase encoding FAO1 YALI0B14014g gene; and b. at least one disrupted acyl-CoA oxidase encoding gene selected from the group consisting of; POX1, POX2, POX3, POX4, POX5, and POX6, wherein the engineered Yarrowia lipolytica cell has reduced fatty alcohol oxidase activity relative to a wild type Yarrowia lipolytica cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
-
13. A genetically engineered Yarrowia lipolytica cell, comprising:
-
a. a disrupted fatty alcohol oxidase encoding FAO1 YALI0B14014g gene; and b. at least one of the following genes disrupted selected from the group consisting of;
phosphatidic acid dephosphohydrolase (PAH1), phospholipid diacylglycerol acyltransferase (LRO1), diacylglycerol acyltransferase (DGA1), and glycerol-3-phosphate acyltransferase (SCT1),wherein the engineered Yarrowia lipolytica cell has reduced fatty alcohol oxidase activity relative to a wild type Yarrowia lipolytica cell. - View Dependent Claims (14, 15, 16)
-
-
17. A genetically engineered Yarrowia lipolytica cell, comprising:
-
a. a disrupted fatty alcohol oxidase encoding FAO1 YALI0B14014g gene; and b. a disrupted peroxisome biogenesis factor 10 protein (PEX10), wherein the engineered Yarrowia lipolytica cell has reduced fatty alcohol oxidase activity relative to a wild type Yarrowia lipolytica cell. - View Dependent Claims (18, 19, 20)
-
Specification