Method and System for Protecting Honey Bees, Bats and Butterflies From Neonicotinoid Pesticides

• US 20190022151A1
• Filed: 09/24/2018
• Published: 01/24/2019
• Est. Priority Date: 11/30/2015
• Status: Active Grant

• Current Assignee: Seed Health, Inc.
• Original Assignee: Joseph E. Kovarik
• Inventors: Kovarik, Joseph E.
• Granted Patent: US 10,568,916 B2
• Time in Patent Office: Days
• CPC Class Codes: A61K 35/74 Bacteria therapeutic use of a bacterial protein A61K38/00
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: C12N 1/00 Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12N 2310/20 involving clustered regularly interspaced short palindromic repeats [CRISPRs]
• CPC Class Codes: C12R 2001/00 Microorganisms ; Processes using microorganisms
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Abstract

A method and system for the treatment of honey bees (Apis mellifera), bats, and butterflies protects them from various life threatening conditions, including Colony Collapse Disorder, white nose syndrome, etc. and in particular, provides honey bees, bats and butterflies with the ability to assimilate and degrade neonicotinoids.

Claims

1. A method for providing a honey bee with the ability to assimilate neonicotinoid pesticides, comprising, inoculating a honey bee with a culture of neonicotinoid degrading bacteria, wherein the neonicotinoid degrading bacteria comprises Ochrobactrum intermedium that has been modified using a clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR associated protein (Cas) system or using a clustered regularly interspaced short palindromic repeats (CRISPR) from prevotella and francisella 1 (Cpf1) nuclease.

2. The method as set forth in claim 1, wherein the neonicotinoid is selected from the group consisting of acetamiprid, clothianidin, dinotefuran, nitenpyram, thiacloprid, and thiamethoxam.

3. The method as set forth in claim 1, wherein the neonicotinoid is acetamiprid.

4. The method as set forth in claim 1, wherein the neonicotinoid is clothianidin.

5. The method as set forth in claim 1, wherein the neonicotinoid is dinotefuran.

6. The method as set forth in claim 1, wherein the neonicotinoid is nitenpyram.

7. The method as set forth in claim 1, wherein the neonicotinoid is thiacloprid.

8. The method as set forth in claim 1, wherein the neonicotinoid is thiamethoxam.

9. A method for providing a honey bee with the ability to assimilate neonicotinoid pesticides, comprising, inoculating a honey bee with a culture of neonicotinoid degrading bacteria, wherein the neonicotinoid degrading bacteria comprises Ochrobactrum intermedium that has been modified using a clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR associated protein (Cas) system.

10. The method as set forth in claim 9, wherein the neonicotinoid is acetamiprid.

11. The method as set forth in claim 9, wherein the neonicotinoid is clothianidin.

12. The method as set forth in claim 9, wherein the neonicotinoid is dinotefuran.

13. The method as set forth in claim 9, wherein the neonicotinoid is nitenpyram.

14. The method as set forth in claim 9, wherein the neonicotinoid is thiacloprid.

15. The method as set forth in claim 9, wherein the neonicotinoid is thiamethoxam.

17. The method as set forth in claim 9, wherein the neonicotinoid is clothianidin.

18. The method as set forth in claim 9, wherein the neonicotinoid is dinotefuran.

19. The method as set forth in claim 9, wherein the neonicotinoid is nitenpyram.

20. The method as set forth in claim 9, wherein the neonicotinoid is thiacloprid.

16. A method for providing a honey bee with the ability to assimilate neonicotinoid pesticides, comprising, inoculating the gut of a honey bee with a culture of microbes that normally inhabit the honey bee gut, said microbes possessing the ability to degrade neonicotinoid pesticides, said microbes comprising neonicotinoid degrading bacteria that have genes from Ochrobactrum intermedium and wherein the neonicotinoid degrading bacteria have been modified using a clustered regularly interspaced short palindromic repeats (CRISPR) from prevotella and francisella 1 (Cpf1) nuclease.