SYNTHETIC METHYLOTROPHS
First Claim
1. A non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the microbe expresses the heterologous MDH, and wherein the heterologous MDH exhibits a Km of no more than 3 mM for methanol.
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Accused Products
Abstract
The present invention provides a non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the MDH is expressed in the microbe, and wherein the MDH exhibits a Km of no more than 3 mM for methanol. Also provided are uses of the non-naturally occurring microbe for oxidizing methanol and producing a metabolite as well as the preparation of the non-naturally occurring microbe.
0 Citations
29 Claims
- 1. A non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the microbe expresses the heterologous MDH, and wherein the heterologous MDH exhibits a Km of no more than 3 mM for methanol.
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Specification