SELECTIVE OXIDATION OF 5-METHYLCYTOSINE BY TET-FAMILY PROTEINS
First Claim
Patent Images
1. A method comprising:
- labeling a hydroxyl group on a hydroxymethylated residue in a nucleic acid to generate a labeled hydroxymethylated residue, wherein said nucleic acid is from an extracellular fluid sample; and
sequencing said nucleic acid comprising said labeled hydroxymethylated residue.
5 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.
11 Citations
20 Claims
-
1. A method comprising:
-
labeling a hydroxyl group on a hydroxymethylated residue in a nucleic acid to generate a labeled hydroxymethylated residue, wherein said nucleic acid is from an extracellular fluid sample; and sequencing said nucleic acid comprising said labeled hydroxymethylated residue. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
-
- 15. A composition comprising a nucleic acid from an extracellular fluid sample, wherein said nucleic acid comprises a covalently labeled hydroxyl group on a hydroxymethylated residue.
Specification