METHOD FOR CULTIVATING CORDYCEPS MILITARIS FRUITING BODY

• US 20190053438A1
• Filed: 09/18/2017
• Published: 02/21/2019
• Est. Priority Date: 08/17/2017
• Status: Active Grant

• Current Assignee: Food Industry Research & Development Institute
• Original Assignee: Food Industry Research & Development Institute
• Inventors: KUO, Hsiao-Ping, LIN, Yi-Sheng, HUANG, Shyue-Tsong, WU, An-Chi, LAI, Jinn-Tsyy
• Granted Patent: US 10,455,778 B2
• Time in Patent Office: Days
• CPC Class Codes: A01G 18/00 Cultivation of mushrooms
• CPC Class Codes: A01G 18/20 Culture media, e.g. compost
• CPC Class Codes: A23F 3/163 Liquid or semi-liquid tea extract preparations, e.g. gels, liquid extracts in solid capsules

Abstract

A method for cultivating Cordyceps militaris fruiting body capable of improving C. militaris fruiting body morphology is provided, wherein rice absorbed with a tea liquid is used as a solid-state culture medium. In comparison to using rice absorbed with water as a solid-state culture medium, the C. militaris fruiting body cultivated by using the solid-state culture medium of the present invention under the same cultivation condition are significantly more robust.

Claims

1. A method for cultivating Cordyceps militaris fruiting body, comprising:

a) providing a seed source, and the seed source being activated C. militaris spores or C. militaris mycelia;

b) providing a solid-state culture medium, wherein the solid-state culture medium comprises rice;

c) inoculating the rice of the solid-state culture medium of step b) with the seed source of step a), and cultivating the same until a fruiting body of C. militaris is formed;

characterized in that;

the rice of the solid-state culture medium of step b) is absorbed with a tea liquid.

2. The method of claim 1, wherein the solid-state culture medium of step b) is provided by a method comprising the steps of:

1) sequentially adding tea leaves, rice and water into a container, wherein a dry weight ratio between the tea leaves and the rice is 0.5;

30 to 3;

30, and a weight ratio of a total dry weight of the tea leaves and the rice to the water is 1;

1 to 1;

0.5; and

2) sterilizing the tea leaves, the rice and the water in the container.

3. The method of claim 1, wherein the solid-state culture medium of step b) is provided by a method comprising the steps of:

i) mixing tea leaves and water;

ii) performing solid-liquid separation on the mixture of step i) to obtain a tea liquid;

iii) mixing rice with the tea liquid of step ii), and sterilizing the resulting mixture of the rice and the tea liquid,

wherein a dry weight ratio between the tea leaves and the rice is 0.5;

30 to 3;

30, and a weight ratio of a total dry weight of the tea leaves and the rice to the water is 1;

1 to 1;

0.5.

4. The method of claim 2;

wherein the tea leaves can be an unfermented tea, a semi-fermented tea or a fermented tea.

5. The method of claim 3;

wherein the tea leaves can be an unfermented tea, a semi-fermented tea or a fermented tea.

6. The method of claim 4;

wherein the tea leaves is an unfermented tea.

7. The method of claim 5;

wherein the tea leaves is an unfermented tea.

8. The method of claim 1, wherein the rice can be brown rice, germ rice or white rice.

9. The method of claim 8, wherein the rice is white rice.

10. The method of claim 2, wherein the tea leaves and the rice added into the container are naturally distributed.

11. The method of claim 3, wherein the water of step i) is hot water having a temperature between 45-99°

C., and a mixing time of the tea leaves and the water is between 1 minute to 1 hour.

12. The method of claim 3, wherein the water of step i) is water having a temperature between 5°

C. to room temperature, and a mixing time of the tea leaves and the water is between 0.5 hour to 48 hours.

13. The method of claim 1, wherein the seed source of step a) is activated C. militaris spores.

14. The method of claim 1, wherein the seed source of step a) is activated C. militaris mycelia.