COMPOSITIONS AND METHODS FOR PHOSPHORAMIDITE AND OLIGONUCLEOTIDE SYNTHESIS
First Claim
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1. A method for purifying a phosphoramidite, comprising steps of:
- a) removing water from a purification medium;
b) contacting the purification medium with a phosphoramidite; and
c) optionally using the purification medium to purify a phosphoramidite.
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Abstract
The present disclosure, among other things, provides technologies for preparing and purifying phosphoramidites for oligonucleotide synthesis.
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Citations
19 Claims
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1. A method for purifying a phosphoramidite, comprising steps of:
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a) removing water from a purification medium; b) contacting the purification medium with a phosphoramidite; and c) optionally using the purification medium to purify a phosphoramidite. - View Dependent Claims (5, 6, 7, 8, 9, 10, 15, 16, 18)
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2. A method for improving recovery rate of a phosphoramidite, comprising steps of:
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a) removing water from a purification medium; b) adding the phosphoramidite to the purification medium; and c) eluting the phosphoramidite from the purification medium with a solvent system; wherein the recovery rate is higher than a reference recovery rate when step a) is absent. - View Dependent Claims (3)
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4. A method for decreasing decomposition of a phosphoramidite when the phosphoramidite contacts a purification medium, comprising:
a) removing water from the purification medium.
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11. A method comprising steps of:
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a) pre-treating a purification medium; b) contacting the purification medium with a phosphoramidite; and b) optionally using the purification medium to purify a phosphoramidite. - View Dependent Claims (12, 13, 14)
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17. A composition comprising:
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a) silica gel pre-treated with a hygroscopic solvent; b) a phosphoramidite.
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19. A method or a composition of any one of embodiments 1-144.
Specification