Systems, Processes, Methods and Machines for Transforming Image Data Into Sizing and Volume Measurements for Tissue
First Claim
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1. An automatic tissue scanning camera (ATSC) system, which comprises, in combination:
- a non-phase contrast microscope;
a polychrome charge-coupled device (CCD) camera;
a horizontally-mounted culture flask;
a trans-illumination means, which exceeds the deep field image size by a factor of at least two; and
an automated islet measurement system (AIMS), comprising tissue volume analysis (TVA) software, the AIMS configured to;
identify a cell in the culture flask, wherein opposing surfaces of the culture flask do not deform the cell;
image the cell with the camera, wherein the cell has a natural, undeformed by a mechanical force, shape, the image comprising pixels;
evaluate a cross-section size of the cell;
measure an optical transmission at each pixel within the cell;
calculate a volume of tissue at each pixel; and
calculate a total volume for the cell.
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Abstract
Automated islet measurement systems (AIMS) in combination with tissue volume analysis (TVA) software effectively gauges volumetric and size-based data to generate heretofore unavailable information regarding, for example, populations of islet cells, stem cells and related desiderata.
4 Citations
16 Claims
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1. An automatic tissue scanning camera (ATSC) system, which comprises, in combination:
- a non-phase contrast microscope;
a polychrome charge-coupled device (CCD) camera;
a horizontally-mounted culture flask;
a trans-illumination means, which exceeds the deep field image size by a factor of at least two; and
an automated islet measurement system (AIMS), comprising tissue volume analysis (TVA) software, the AIMS configured to;
identify a cell in the culture flask, wherein opposing surfaces of the culture flask do not deform the cell;
image the cell with the camera, wherein the cell has a natural, undeformed by a mechanical force, shape, the image comprising pixels;
evaluate a cross-section size of the cell;
measure an optical transmission at each pixel within the cell;
calculate a volume of tissue at each pixel; and
calculate a total volume for the cell. - View Dependent Claims (2, 3, 4, 5, 6)
- a non-phase contrast microscope;
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7. A method of measuring the volume of a live unfixed cell with a natural, undeformed by a mechanical force, shape comprising the steps of:
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identifying a live unfixed cell in a flask, wherein opposing surfaces of the flask do not deform the live unfixed cell; visualizing the live unfixed cell in the flask through a lens of a non-phase contrast microscope connected to a polychrome charge-coupled device (CCD) camera; illuminating the live unfixed cell with a trans-illumination means, which exceeds deep field image size by a factor of at least two; imaging the live unfixed cell with the camera, wherein the live unfixed cell has a natural, undeformed by a mechanical force, shape;
the image comprising pixels;evaluating a cross-section size of the live unfixed cell; measuring an optical transmission at each pixel within the live unfixed cell; calculating a volume of tissue at each pixel; and calculating a total volume for the live unfixed cell with an automated cell measurement system, comprising tissue volume analysis software. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification