METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
62 Citations
60 Claims
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1-30. -30. (canceled)
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31. A method for nucleic acid processing, comprising:
- providing a microwell array comprising (1) a plurality of polynucleotide molecules, (2) a plurality of beads, and (3) a plurality of barcode molecules, wherein said microwell array comprises 1,000 microwells, wherein said plurality of barcode molecules comprises a plurality of barcode sequences that are different across said 1,000 microwells, and
wherein a microwell of said microwell array comprises;
(i) a polynucleotide molecule of said plurality of polynucleotide molecules, and (ii) a barcode molecule of said plurality of barcode molecules, wherein said barcode molecule is attached to a bead of said plurality of beads and comprises a barcode sequence of said plurality of barcode sequences. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
- providing a microwell array comprising (1) a plurality of polynucleotide molecules, (2) a plurality of beads, and (3) a plurality of barcode molecules, wherein said microwell array comprises 1,000 microwells, wherein said plurality of barcode molecules comprises a plurality of barcode sequences that are different across said 1,000 microwells, and
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49. A method for biological material processing, comprising:
- providing a microwell array comprising (1) a plurality of beads, (2) a plurality of cells, and (3) a plurality of barcode molecules, wherein said microwell array comprises 1,000 microwells, wherein said plurality of barcode molecules comprises a plurality of barcode sequences that are different across said 1,000 microwells, and
wherein a microwell of said microwell array accommodates a volume of less than about 5 nanoliters and comprises;
(i) a cell of said plurality of cells; and
(ii) a bead of said plurality of beads. - View Dependent Claims (50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60)
- providing a microwell array comprising (1) a plurality of beads, (2) a plurality of cells, and (3) a plurality of barcode molecules, wherein said microwell array comprises 1,000 microwells, wherein said plurality of barcode molecules comprises a plurality of barcode sequences that are different across said 1,000 microwells, and
Specification