Method and System for Protecting Honey Bees, Bats and Butterflies From Neonicotinoid Pesticides

• US 20190142934A1
• Filed: 01/14/2019
• Published: 05/16/2019
• Est. Priority Date: 11/30/2015
• Status: Active Grant

• Current Assignee: Seed Health, Inc.
• Original Assignee: Joseph E. Kovarik
• Inventors: Kovarik, Joseph E.
• Granted Patent: US 10,675,347 B2
• Time in Patent Office: Days
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61K 2039/541 Mucosal route
• CPC Class Codes: A61K 2039/544 to the airways intranasal A61K2039/543
• CPC Class Codes: A61K 2039/55 characterised by the host/recipient, e.g. newborn with maternal antibodies
• CPC Class Codes: A61K 2039/55583 Polysaccharides
• CPC Class Codes: A61K 2039/577 tolerising response
• CPC Class Codes: A61K 2039/58 raising an immune response against a target which is not the antigen used for immunisation
• CPC Class Codes: A61K 2039/70 Multivalent vaccine
• CPC Class Codes: A61K 35/74 Bacteria therapeutic use of a bacterial protein A61K38/00
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61K 35/742 Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis
• CPC Class Codes: A61K 39/0008 Antigens related to auto-immune diseases; Preparations to induce self-tolerance
• CPC Class Codes: A61K 39/35 Allergens
• CPC Class Codes: A61K 39/39 characterised by the immunostimulating additives, e.g. chemical adjuvants
• CPC Class Codes: A61K 39/395 Antibodies agglutinins A61K38/36 ; as drug carriers A61K47/50; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 9/0034 Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
• CPC Class Codes: A61K 9/0043 Nose
• CPC Class Codes: A61K 9/007 : Pulmonary tract; Aromatherapy
• CPC Class Codes: C07K 16/00 : Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002

Abstract

A method and system for the treatment of honey bees (Apis mellifera), bats, and butterflies protects them from various life threatening conditions, including Colony Collapse Disorder, white nose syndrome, etc. and in particular, provides honey bees, bats and butterflies with the ability to assimilate and degrade pesticides such as neonicotinoids and fipronil.

Claims

1. A method for providing a honey bee with the ability to assimilate pesticides, comprising, inoculating a honey bee with a culture of one of a neonicotinoid degrading bacteria and a fipronil degrading bacteria, wherein the neonicotinoid degrading bacteria comprises Ochrobactrum intermedium, and the fipronil degrading bacteria comprises at least one of Bacillus thuringiensis, Paracoccus denitrificans, and Stenotrophomonas acidaminiphila.

2. The method as set forth in claim 1, wherein the neonicotinoid degraded by the neonicotinoid bacteria is selected from the group consisting of acetamiprid, clothianidin, dinotefuran, nitenpyram, thiacloprid, and thiamethoxam.

3. The method as set forth in claim 1, wherein the culture comprises one of a neonicotinoid degrading bacteria and a fipronil degrading bacteria, that has been modified using a clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR associated protein (Cas) system or using a clustered regularly interspaced short palindromic repeats (CRISPR) from prevotella and francisella 1 (Cpf1) nuclease.

4. The method as set forth in claim 1, wherein the culture comprises a fipronil degrading bacteria comprises one of Paracoccus denitrificans and Stenotrophomonas acidaminiphila.

5. The method as set forth in claim 1, wherein the culture comprises a fipronil degrading bacteria that comprises Stenotrophomonas acidaminiphila.

6. The method as set forth in claim 1, wherein the culture comprises a fipronil degrading bacteria that comprises Bacillus thuringiensis.

7. The method as set forth in claim 1, wherein the neonicotinoid pesticide is thiacloprid.

8. The method as set forth in claim 1, wherein the neonicotinoid pesticide is thiamethoxam.

9. A method for providing a honey bee with the ability to assimilate pesticides, comprising, inoculating a honey bee with a culture of pesticide degrading bacteria, wherein the pesticide is selected from the group consisting of fipronil, and a neonicotinoid pesticide, said neonicotinoid selected from the group consisting of acetamiprid, clothianidin, dinotefuran, nitenpyram, thiacloprid, and thiamethoxam, said culture including one of Ochrobactrum intermedium and Paracoccus denitrificans.

10. The method as set forth in claim 9, wherein the culture has been modified using a clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR associated protein (Cas) system to facilitate degradation of at least one pesticide, said pesticide selected from the group consisting of fipronil and a neonicotinoid pesticide.

11. The method as set forth in claim 9, wherein the culture further comprises one of Stenotrophomonas acidaminiphila, Bacillus thuringiensis, and Paracoccus denitrificans.

12. The method as set forth in claim 9, wherein the culture further comprises one of Stenotrophomonas acidaminiphila and Bacillus thuringiensis.

13. The method as set forth in claim 9, wherein the neonicotinoid pesticide is nitenpyram.

14. The method as set forth in claim 9, wherein the neonicotinoid pesticide is thiacloprid.

15. The method as set forth in claim 9, wherein the neonicotinoid pesticide is thiamethoxam.

17. The method as set forth in claim 9, wherein the culture further comprises one of Stenotrophomonas acidaminiphila and Bacillus thuringiensis.

18. The method as set forth in claim 9, wherein the neonicotinoid pesticide is dinotefuran.

19. The method as set forth in claim 9, wherein the neonicotinoid pesticide is nitenpyram.

20. The method as set forth in claim 9, wherein the neonicotinoid pesticide is thiacloprid.

16. A method for providing a honey bee with the ability to assimilate pesticides, comprising, inoculating the gut of a honey bee with a culture of microbes that normally inhabit the honey bee gut, said microbes possessing the ability to degrade pesticides, said microbes comprising one of neonicotinoid degrading bacteria that have genes from Ochrobactrum intermedium, and fipronil degrading bacteria that have genes from Paracoccus denitrificans, wherein the microbes have been modified using a clustered regularly interspaced short palindromic repeats (CRISPR) from prevotella and francisella 1 (Cpf1) nuclease.