PRRS VIRUS VARIANT, EUROPEAN PRRS VIRUS CDNA CLONE, AND USES THEREOF

• US 20190151432A1
• Filed: 06/01/2018
• Published: 05/23/2019
• Est. Priority Date: 12/20/2013
• Status: Active Grant

• Current Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Inventors: Gallei, Andreas, Keller, Christoph, Schacht, Erik, Herrel, Marieke
• Granted Patent: US 10,639,364 B2
• Time in Patent Office: Days
• CPC Class Codes: A61K 2039/5254 avirulent or attenuated
• CPC Class Codes: A61K 2039/552 Veterinary vaccine
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/86 Viral vectors
• CPC Class Codes: C12N 2770/10021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/10034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2770/10043 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12Q 1/02 involving viable microorganisms
• CPC Class Codes: G01N 2333/08 RNA viruses

Abstract

The present invention belongs to the field of animal health and provides means to study Porcine Reproductive and Respiratory Syndrome (PRRS), a viral disease affecting swine, and for the development of vaccines, therapeutics and diagnostics for the prophylaxis, treatment and diagnosis of PRRS. In a first consideration, the invention relates to a new PRRS virus variant. and, in a second consideration, to a nucleic acid sequence which comprises the genome of an infectious genotype I (EU) PRRS virus clone. Based on this, new PRRS vaccine candidates with improved properties are provided.

Claims

1. A genotype I Porcine Reproductive and Respiratory Syndrome (PRRS) virus whose genome is encoded by a nucleic acid molecule comprising a sequence selected from SEQ ID NOS:

49, 56, 57, and 58.

2. The virus of claim 1, wherein the nucleic acid comprises the sequence of SEQ ID NO:

49.

3. The virus of claim 1, wherein the nucleic acid comprises the sequence of SEQ ID NO:

56.

4. The virus of claim 1, wherein the nucleic acid comprises the sequence of SEQ ID NO:

57.

5. The virus of claim 1, wherein the nucleic acid comprises the sequence of SEQ ID NO:

58.

6. A vaccine comprising the virus according to claim 1.

7. The vaccine of claim 6, further comprising a pharmaceutically acceptable excipient.

8. The vaccine of claim 7, wherein the pharmaceutically acceptable excipient is selected from a solvent, a dispersion media, an adjuvant, a stabilizing agent, a diluent, a preservative, an antibacterial agent, an antifungal agent, an isotonic agent, and an adsorption delaying agent.

9. The vaccine of claim 6, wherein the vaccine comprises 10⁴ to 10⁶ particles of the virus per dose.

10. The vaccine of claim 6, wherein the nucleic acid comprises the sequence of SEQ ID NO:

49.

11. The vaccine of claim 6, wherein the nucleic acid comprises the sequence of SEQ ID NO:

56.

12. The vaccine of claim 6, wherein the nucleic acid comprises the sequence of SEQ ID NO:

57.

13. The vaccine of claim 6, wherein the nucleic acid comprises the sequence of SEQ ID NO:

58.

14. A method for treating and/or prophylaxis of PRRS in swine, comprising administering to the swine the vaccine according to claim 6.

15. The method of claim 14, wherein the vaccine is administered by an intranasal, an intramuscular, an oral, or an intrauterine route.

16. The method of claim 14, wherein the administration induces a protective immune response against PRRS in the swine that reduces and/or prevents PRRS symptoms.

17. The method of claim 14, wherein the nucleic acid comprises the sequence of SEQ ID NO:

56.

18. The method of claim 14, wherein the nucleic acid comprises the sequence of SEQ ID NO:

58.

19. A method for differentiating between infection and vaccination of a swine, comprising detecting for a vaccine marker in the swine that differentiates between native infection with PRRS and the vaccine according to claim 1.

20. The method of claim 19, wherein the vaccine marker is a deletion or insertion marker that permits serological and/or sequence differentiation.