PROTEIN-INDUCED PLURIPOTENT CELL TECHNOLOGY AND USES THEREOF
First Claim
1. A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique;
- repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells;
moving the reprogrammed cells into a feeder-free medium for expansion; and
expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells.
1 Assignment
0 Petitions
Accused Products
Abstract
A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPCS cells formed using this method and uses thereof.
-
Citations
14 Claims
-
1. A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique;
-
repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells; moving the reprogrammed cells into a feeder-free medium for expansion; and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
-
Specification