AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS
First Claim
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1. An automated stand-alone multi-module cell editing instrument for performing inducible nuclease editing comprising:
- a receptacle configured to receive cells;
one or more receptacles configured to receive nucleic acids, wherein the nucleic acids comprise an inducible nuclease editing system;
a growth module for growing cells for transformation;
a transformation module configured to introduce the nucleic acids into the cells;
a nuclease-directed editing module configured to activate the inducible nuclease editing system and allow the introduced nucleic acids to edit nucleic acids in the cells;
a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; and
an automated liquid handling system to move cells from the cell receptacle to the growth module, from the growth module to the transformation module and from the transformation module to the nuclease-directed editing module without user intervention.
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Abstract
In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
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Citations
20 Claims
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1. An automated stand-alone multi-module cell editing instrument for performing inducible nuclease editing comprising:
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a receptacle configured to receive cells; one or more receptacles configured to receive nucleic acids, wherein the nucleic acids comprise an inducible nuclease editing system; a growth module for growing cells for transformation; a transformation module configured to introduce the nucleic acids into the cells; a nuclease-directed editing module configured to activate the inducible nuclease editing system and allow the introduced nucleic acids to edit nucleic acids in the cells; a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; and an automated liquid handling system to move cells from the cell receptacle to the growth module, from the growth module to the transformation module and from the transformation module to the nuclease-directed editing module without user intervention. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. An automated stand-alone multi-module cell editing instrument for performing inducible nuclease editing comprising:
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a receptacle configured to receive cells; a nucleic acid assembly module configured to assemble a vector backbone and an editing cassette, wherein the editing cassette comprises one or more inducible components of the inducible editing system, and wherein the nucleic acid assembly module is configured to accept and assemble nucleic acids to facilitate the desired genome editing events in the cells; a growth module configured to grow the cells; a transformation module comprising an electroporator to introduce assembled nucleic acids into the cells; a nuclease-directed editing module configured to activate the inducible nuclease editing system and allow the assembled nucleic acids to edit nucleic acids in the cells; a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a script; and an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the transformation module, and from the transformation module to the nuclease-directed editing module, as well as from the nucleic acid assembly module to the transformation module, all without user intervention. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20)
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Specification