PREPARATION METHOD OF GYNOSTEMMA PENTAPHYLLUM LEAVES EXTRACT FOR INCREASING SMALL MOLECULAR EFFECTIVE SAPONIN CONTENTS, AND DECREASING BENZOPYRENE, AND GYNOSTEMMA PENTAPHYLLUM EXTRACT PREPARED BY THE METHOD THEREOF

• US 20190201468A1
• Filed: 01/04/2018
• Published: 07/04/2019
• Est. Priority Date: 01/04/2018
• Status: Active Grant

• Current Assignee: BTC Corp (Capgemini SE)
• Original Assignee: BTC Corp (Capgemini SE)
• Inventors: KIM, Tae Young, MOON, Joo Myung, KYONG, Su Hyun, KIM, Yoon Hee
• Granted Patent: US 10,639,343 B2
• Time in Patent Office: Days
• CPC Class Codes: A61K 2236/17 involving drying, e.g. sun-drying or wilting
• CPC Class Codes: A61K 2236/33 involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
• CPC Class Codes: A61K 2236/331 using water, e.g. cold water, infusion, tea, steam distillation or decoction
• CPC Class Codes: A61K 36/424 Gynostemma

Abstract

A preparation method of Gynostemma Pentaphyllus leaves extracts with increased amount of small molecular effective saponin and decreased benzopyrene includes the steps of drying fresh Gynostemma Pentaphyllus leaves after heating, treating the dried Gynostemma Pentaphyllus leaves with steam, adding water of 1 to 100-fold volume to the above Gynostemma Pentaphyllus leaves and then preparing a hot water extract by carrying out a hot water extraction at 100-180 V, 0.5-10 atm for 1-120 hours, adding C₁-C₄ lower alcohol of 1 to 100-fold volume to the residual remained after the hot water extraction and then preparing an alcohol extract of the residual from the hot water extract by carrying out an alcohol extraction at 50-100° C. for 1-4 hours, and mixing the hot water extract and the alcohol extract, and then filtering and concentrating the mixture.

Claims

1. A preparation method of Gynostemma Pentaphyllus leaves extracts with increased amount of small molecular effective saponin and decreased benzopyrene comprising the steps of:

(1) drying fresh Gynostemma Pentaphyllus leaves after heating;

(2) treating the dried Gynostemma Pentaphyllus leaves with steam;

(3) adding water of 1 to 100-fold volume to the above Gynostemma Pentaphyllus leaves and then preparing a hot water extract by carrying out a hot water extraction at 100-180°

C., 0.5-10 atm for 1-120 hours;

(4) adding C₁-C₄ lower alcohol of 1 to 100-fold volume to the residual remained after the hot water extraction and then preparing an alcohol extract of the residual from the hot water extract by carrying out an alcohol extraction at 50-100 for 1-4 hours; and

mixing the hot water extract of step (3) and the alcohol extract of step (4), and then filtering and concentrating the mixture.

2. The preparation method of Gynostemma Pentaphyllus leaves extracts with increased amount of the small molecular effective saponin and decreased benzopyrene according to claim 1,

characterized in that the heating in step (1) is carried out by roasting fresh leaves of Gynostemma Pentaphyllus at 90-180°

C. for 1-120 hours using an electric heater, and then drying the Gynostemma Pentaphyllus leaves.

3. The preparation method of Gynostemma Pentaphyllus leaves extracts with increased amount of the small molecular effective saponin and decreased benzopyrene according to claim 1,

characterized in that the heating in step (1) is carried out by steaming fresh leaves of Gynostemma Pentaphyllus at 90-180°

C. for 1-120 hours, and then drying the Gynostemma Pentaphyllus leaves.

4. The preparation method for Gynostemma Pentaphyllus leaves extracts with increased amount of the small molecular effective saponin and decreased benzopyrene according to claim 1,

characterized in that the steam treatment in step (2) is carried out at 100-130°

C. for 0.5-10 hours.

5. The preparation method for Gynostemma Pentaphyllus leaves extracts with increased amount of the small molecular effective saponin and decreased benzopyrene according to claim 1,

characterized in that the process of roasting the above steam-treated Gynostemma Pentaphyllus leaves at 90-180°

C. for 1-120 hours using an electric heater, drying the leaves, and treating them with steam at 100-130°

C. for 0.5-10 hours is repeatedly carried out 1 to 5 times after step (1) and step (2) are completed.

6. The preparation method for Gynostemma Pentaphyllus leaves extracts with increased amount of the small molecular effective saponin and decreased benzopyrene according to claim 1,

characterized in that the C₁-C₄ lower alcohol in the preparation step of the alcohol extract is one or more respectively selected from methanol, ethanol, propanol, isopropanol, and butanol.

7. The preparation method for Gynostemma Pentaphyllus leaves extracts with increased amount of the small molecular effective saponin and decreased benzopyrene according to claim 1,

characterized in that the lower alcohol in the preparation step of the alcohol extract is an alcohol aqueous solution of 20-80% (v/v), respectively.

8. The preparation method for Gynostemma Pentaphyllus leaves extracts with increased amount of the small molecular effective saponin and decreased benzopyrene according to claim 1,

characterized in that the pH of the solvent used for the extraction is in the range from pH 0 to pH 7.

9. The preparation method for Gynostemma Pentaphyllus leaves extracts with increased amount of the small molecular effective saponin and decreased benzopyrene according to claim 7,

characterized in adding one or more from citric acid, butyric acid, hydrochloric acid, trifluoroacetic acid, trichloroacetic acid, acetic acid, vitamin C, malic acid, lactic acid, succinic acid, alginate to regulate the pH of solvent.

10. The Gynostemma Pentaphyllus leaves extracts prepared by the method according to claim 1,

characterized in additionally comprising 0.01˜

7 mg/g of ginsenoside Rg3, 1.5˜

70 mg/g of gypenoside L, 1.5˜

70 mg/g of gypenoside LI.

11. The Gynostemma Pentaphyllus leaves extracts prepared by the method according to claim 1,

characterized in comprising 10 or less ppb benzopyrene.

18. A composition comprising the Gynostemma Pentaphyllus leaves extract of claim 10 as an active ingredient.

19. A composition comprising the Gynostemma Pentaphyllus leaves extract of claim 11 as an active ingredient.

12. The Gynostemma Pentaphyllus leaves extracts characterized in comprising 10 or less ppb benzopyrene.

13. The Gynostemma Pentaphyllus leaves extracts according to claim 12, characterized in additionally comprising 0.01˜

7 mg/g of ginsenoside Rg3, 1.5˜

70 mg/g of gypenoside L, 1.5˜

70 mg/g of gypenoside LI.

14. The Gynostemma Pentaphyllus leaves extracts according to claim 12 in a form of dried power.

20. A composition comprising the Gynostemma Pentaphyllus leaves extract of claim 12 as an active ingredient.

15. The Gynostemma Pentaphyllus leaves extracts characterized in comprising 0.01˜

7 mg/g of ginsenoside Rg3, 1.5˜

70 mg/g of gypenoside L, 1.5˜

70 mg/g of gypenoside LI.

16. The Gynostemma Pentaphyllus leaves extracts according to claim 15 characterized in comprising 10 or less ppb benzopyrene.

17. The Gynostemma Pentaphyllus leaves extracts according to claim 15 in a form of dried power.

21. A composition comprising the Gynostemma Pentaphyllus leaves extract of claim 15 as an active ingredient.