TOBACCO PLANT AND METHOD FOR MANUFACTURING SAME
First Claim
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1. A tobacco plant in which a mutation is introduced into a genome, which mutation causes functional suppression of any of the following genes (1) through (4):
- (1) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 1 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 3;
(2) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 9 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 11;
(3) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 13 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 15; and
(4) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 17 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 19,the functional suppression suppressing development of, of all of axillary buds, secondary axillary buds which develop after removal of primary axillary buds,the functional suppression being a decrease in abundance of the polypeptide in comparison with a wild-type plant,the functional suppression being promotion of degradation of an mRNA transcribed from any of the above genes (1) through (4),the mutation being insertion, into an outside of a region in which any of the above genes (1) through (4) is present, of a polynucleotide expressing a factor which promotes the degradation of the mRNA, andthe factor being an antisense RNA molecule or an RNAi molecule.
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Abstract
The present invention provides a tobacco plant which is suitable for cultivation for harvesting leaf tobaccos. The present invention includes (i) a tobacco plant in which a mutation for suppressing the development of axillary buds is introduced and (ii) a method of producing the tobacco plant.
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Citations
30 Claims
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1. A tobacco plant in which a mutation is introduced into a genome, which mutation causes functional suppression of any of the following genes (1) through (4):
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(1) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 1 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 3; (2) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 9 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 11; (3) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 13 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 15; and (4) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 17 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 19, the functional suppression suppressing development of, of all of axillary buds, secondary axillary buds which develop after removal of primary axillary buds, the functional suppression being a decrease in abundance of the polypeptide in comparison with a wild-type plant, the functional suppression being promotion of degradation of an mRNA transcribed from any of the above genes (1) through (4), the mutation being insertion, into an outside of a region in which any of the above genes (1) through (4) is present, of a polynucleotide expressing a factor which promotes the degradation of the mRNA, and the factor being an antisense RNA molecule or an RNAi molecule. - View Dependent Claims (3, 10, 19, 23, 27)
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2. A tobacco plant in which a mutation is introduced into a genome, which mutation causes functional suppression of any of the following genes (1) through (5):
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(1) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 1 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 3; (2) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 5 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 7; (3) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 9 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 11; (4) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 13 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 15; and (5) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 17 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 19, the functional suppression suppressing development of, of all of axillary buds, secondary axillary buds which develop after removal of primary axillary buds, and the mutation being introduced into any of the above genes (1) through (5). - View Dependent Claims (4, 5, 6, 7, 8, 9, 11, 20, 24, 28)
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12. A method of producing a tobacco plant, comprising the step of:
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(a) introducing a mutation that causes functional suppression of any of the following genes (1) through (4); (1) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 1 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 3; (2) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 9 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 11; (3) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 13 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 15; and (4) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 17 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 19, the functional suppression suppressing development of, of all of axillary buds, secondary axillary buds which develop after removal of primary axillary buds, the step (a) including inserting, into an outside of a region in which any of the above genes (1) through (4) is present, a polynucleotide expressing a factor which promotes degradation of an mRNA transcribed from any of the above genes (1) through (4), and the factor being an antisense RNA molecule or an RNAi molecule. - View Dependent Claims (14, 16, 21, 25, 29)
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13. A method of producing a tobacco plant, comprising the step of:
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(a) introducing a mutation that causes functional suppression of any of the following genes (1) through (5); (1) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 1 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 3; (2) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 5 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 7; (3) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 9 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 11; (4) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 13 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 15; and (5) at least one of a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 17 and a gene containing, as a coding region, a polynucleotide encoding a polypeptide having a sequence identity of 90% or higher with an amino acid sequence represented by SEQ ID NO. 19, the functional suppression suppressing development of, of all of axillary buds, secondary axillary buds which develop after removal of primary axillary buds, and the step (a) including introducing the mutation into any of the above genes (1) through (5). - View Dependent Claims (15, 17, 18, 22, 26, 30)
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Specification