Bioaerosol Detector Having Safeguards

• US 20190226973A1
• Filed: 04/05/2018
• Published: 07/25/2019
• Est. Priority Date: 01/23/2018
• Status: Active Grant

• Current Assignee: Elric Saaski
• Original Assignee: CBRN International, Ltd.
• Inventors: SAASKI, ELRIC, YACOBI, DOR
• Granted Patent: US 10,444,137 B2
• Time in Patent Office: Days
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/0205 by optical means
• CPC Class Codes: G01N 15/0211 Investigating a scatter or diffraction pattern
• CPC Class Codes: G01N 15/06 Investigating concentration of particle suspensions by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10
• CPC Class Codes: G01N 15/075 by optical means
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/1409 Handling samples, e.g. injecting samples
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/0046 in gas, e.g. smoke
• CPC Class Codes: G01N 2015/019 Biological contaminants; Fouling
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

A method of detecting bioaerosols, including detecting particles and estimating a particle size for each detected particle and determining a fluorescence strength for each detected particle. Comparing particle size and fluorescence strength for each detected particle to arrive at a normalized fluorescence strength per particle and comparing normalized fluorescence strengths over a time period to a maximum threshold, to detect highly fluorescent manmade substances.

Claims

1. A bioaerosol detector, comprising:

a. a housing defining a chamber;

b. an air stream injection assembly, producing an airstream with entrained particles having an average velocity within a transverse extent, in said chamber;

c. a light source, producing a light beam that intersects and crosses said air stream;

d. a light detection assembly, configured and positioned to detect light generated by scattering of light from said light beam, by particles in said air stream;

e. a digitizer, which produces a sequence of first digital values, representative of light detection, responsive to said light detection assembly;

f. a data processor that repeatedly calculates a background noise level, responsive to said sequence of first digital values, and which is responsive to a high density of small particles condition wherein said noise level rises due to small particles in said air stream that are not detected and are sufficiently numerous to raise the noise level, thereby obscuring said particles from detection, by detecting a condition in which said noise level has risen above a threshold.

2. The bioaerosol detector of claim 1, wherein:

a. said light source is an ultraviolet light source;

b. said light detection assembly is a scattered light detection assembly, including a short pass filter, and which detects shorter wavelength light; and

c. further comprising a fluorescence light detection assembly, including a long wavelength filter, and which detects longer wavelength light indicative of light that has been created by biofluorescence, from said particles in said air stream.

3. The bioaerosol detector of claim 2, wherein a comparison of fluorescent returns and scattering returns is performed for a time period in which a high density of undetected small particles condition is detected.

4. The bioaerosol detector of claim 1, wherein said detector further includes a human perceptible notification device and wherein said device issues a human perceptible notification when a high density of small particles condition is detected.

5. The bioaerosol detector of claim 1, wherein said background noise level is responsive to said sequence of first digital values by being based on a sequence of summed digital values, each summed digital value being a summation of a set of first digital values.

6. A bioaerosol detector, comprising:

a. a housing defining a chamber;

b. an air stream injection assembly, producing an airstream with entrained particles having an average velocity within a transverse extent, in said chamber;

c. a light source, producing a light beam that intersects with and crosses said air stream;

d. a photon detection assembly, including an optical train and a photon detector, and being configured and positioned to generate an output pulse in response to a photon encounter, from light emitted from said light source and scattered by a particle in said air stream;

e. a digitizer, responsive to said photon detector, to produce a sequence of first digital values, each first digital value representing detections of output pulses from said photon detection assembly, per a unit of time duration;

f. a photon detector continuous high state sensor, also responsive to said photon detection assembly, and adapted to detect a continuous high output state, thereby indicating that a flood of photons is preventing said photon detector from transitioning into a low state between photon encounters.

7. The bioaerosol detector of claim 6, wherein said digitizer detects output pulses by detecting rising edges.

8. The bioaerosol detector of claim 6, wherein said digitizer detects output pulses by detecting falling edges.

9. The bioaerosol detector of claim 6, wherein:

a. said light source is an ultraviolet light source;

b. said light detection assembly is a scattered light detection assembly, including a short pass filter, and which detects shorter wavelength light; and

c. further comprising a fluorescence light detection assembly, including a long wavelength filter, and which detects longer wavelength light indicative of light that has been created by biofluorescence from said particles in said air stream.

10. A particle detector, comprising:

a. a housing defining a chamber;

b. an air stream injection assembly, producing an airstream, having a transverse extent, in said chamber;

c. a light source, producing a light beam that intersects with and crosses said air stream;

d. a light detection assembly, configured and positioned to detect scattering of light from said light beam, by particles in said air stream;

e. a digitizer, responsive to said light detection assembly, to produce a sequence of first digital values, each first digital value representing light detected per a unit of time duration;

f. a data processing assembly, computing a background noise estimation, responsive to said sequence of digital values, and repeatedly determining if interior surfaces of said light detection assembly have become degraded by adherent particles by analyzing changes to said background noise estimation over time to determine if an increase in background noise above a background noise increase threshold has taken place over a time period longer than a time period threshold.

11. The particle detector of claim 10, wherein

a. said light source is an ultraviolet light source and

b. wherein said light detection assembly is a scattered light detection assembly, including a short pass filter, and which detects shorter wavelength light;

and

c. further comprising a fluorescence light detection assembly, including a long wavelength filter, and which detects longer wavelength light indicative of light that has been created by biofluorescence from said particles in said air stream.

12. The particle detector of claim 10, wherein the background noise level is assessed for a period of greater than an hour, to determine if there has been a degradation of interior surfaces above a threshold amount.

13. The particle detector of claim 10, wherein said background noise level is responsive to said sequence of first digital values by being based on a sequence of summed digital values, each summed digital value being a summation of a set of first digital values.

14. A method of detecting bioaerosols, comprising:

a. detecting particles and estimating a particle size for each detected particle;

b. determining a fluorescence strength for each detected particle;

c. comparing particle size and fluorescence strength for each detected particle to arrive at a normalized fluorescence strength per particle;

d. comparing normalized fluorescence strengths over a time period to a maximum threshold, to detect highly fluorescent manmade substances.

15. The method of claim 14, wherein a human perceptible notification device issues an advisory, upon the detection of a highly fluorescent manmade substance.

16. The method of claim 14, wherein said threshold is set in reference to the highest level of fluorescence return, relative to particle size, expected from a threatening fluorescent particle.

17. The method of claim 14, wherein said particle detection is performed by illuminating particles in an air stream, passing through a chamber, with ultraviolet light, and detecting and digitizing a resultant light signal, and wherein said fluorescence strength determination is performed by long pass filtering, detecting and digitizing a resultant light signal, and wherein said steps of comparing are performed digitally in a digital computer.

18. A bioaerosol detector, comprising:

a. a housing defining a chamber;

b. an air stream injection assembly, producing an airstream with entrained particles having an average velocity within a transverse extent, in said chamber;

c. an ultraviolet light source, producing a light beam that intersects said air stream;

d. a scattering light detection assembly, configured and positioned to detect light generated by scattering of light from said light beam, by particles in said air stream, and including a first lens that has a surface facing said chamber;

e. a fluorescence light detection assembly, including a long wavelength filter, and which detects longer wavelength light indicative of light that has been created by biofluorescence, from said particles in said air stream, and including a second lens that has a surface facing said chamber; and

f. wherein said scattering light detection assembly can be removed as a single unit, thereby permitting easy access to said first lens, to permit cleaning of said lens.

19. The bioaerosol detector of claim 18, wherein said fluorescence light detection assembly can be removed as a single unit, thereby permitting easy access to said second lens, to permit cleaning of said lens.

20. The bioaerosol detector of claim 18, wherein said scattering light detection assembly is bolted on to said housing, and may be removed by undoing bolts.