METHOD FOR LIBERATING SUGAR CHAIN FROM GLYCOPROTEIN
First Claim
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1. A method for liberating sugar chains from a glycoprotein, comprising:
- a step of brining a reaction solution which contains hydroxylamines (a) and a basic reagent (b) into contact with the glycoprotein to obtain a mixed solution of the sugar chains liberated from the glycoprotein and the reaction solution.
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Abstract
Provided is a method for liberating sugar chains from a glycoprotein, including: a step of brining a reaction solution which contains hydroxylamines (a) and a basic reagent (b) into contact with the glycoprotein to obtain a mixed solution of sugar chains liberated from the glycoprotein and the reaction solution.
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21 Claims
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1. A method for liberating sugar chains from a glycoprotein, comprising:
a step of brining a reaction solution which contains hydroxylamines (a) and a basic reagent (b) into contact with the glycoprotein to obtain a mixed solution of the sugar chains liberated from the glycoprotein and the reaction solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A kit for liberating sugar chains from a glycoprotein, comprising:
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hydroxylamines (a); and a basic reagent (b). - View Dependent Claims (13, 14, 15)
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16. A kit for liberating sugar chains from a glycoprotein, comprising:
a basic reagent (b) and amines (c) for being used by combining with hydroxylamines (a). - View Dependent Claims (17, 18)
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19. A device which liberates sugar chains from a glycoprotein, comprising:
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a container holding portion which holds a container in which a sample containing a glycoprotein is accommodated; and a reagent introduction unit which introduces a reagent into the container, wherein the reagent introduction unit includes a reaction solution introduction unit which introduces a reaction solution containing hydroxylamines (a) and a basic reagent (b) into the container. - View Dependent Claims (20, 21)
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Specification